Molecular characterization of Candida dubliniensis and Candida albicans in the oral cavity of drug abusers using duplex polymerase chain reaction
محل انتشار: سرطان معده، دوره: 4، شماره: 1
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 51
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شناسه ملی سند علمی:
JR_CUMM-4-1_003
تاریخ نمایه سازی: 11 آذر 1402
چکیده مقاله:
Background and Purpose: Candida dubliniensis is closely related to the most pathogenic and prevalent yeast, namely C. albicans. Candida species can opportunistically overgrow in vulnerable individuals and cause a variety of diseases. The current study aimed to identify and isolate C. dubliniensis species present in the Candida albicans species complex identified in the oral cavity of drug abusers. Materials and Methods: This study was conducted on ۵۳ strains of C. albicans species complex, isolated from the oral mucosa of drug abusers in Isfahan, Iran. DNA extraction was accomplished through boiling procedure. Duplex polymerase chain reaction (PCR) was performed to amplify ITS۱-۵.۸S-ITS۲ region using four specific primers. Fungal species were identified based on the difference in the size of the bands created in the Agarose gel. Results: Out of the ۵۳ isolates under study, ۳۰ (۵۶.۶%) and ۱۴ (۲۶.۴%) samples were identified as C. albicans and C. dubliniensis, respectively. In the remaining ۹ samples (۱۷%), both types of Candida species were confirmed. Conclusion: The findings of the present study revealed the presence of a noticeable amount of C. dubliniensis in the oral cavity of drug abusers. Therefore, the probable presence of this fungus should be considered during the examination of oral infection among this group. To date, no research has directly investigated this issue in Iran.
کلیدواژه ها:
نویسندگان
Parastoo Hassani Abharian
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Parvin Dehghan
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Peyman Hassani Abharian
Department of Cognitive Rehabilitation, Institute for Cognitive Sciences Studies, Tehran, Iran
Sepideh Tolouei
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran