In-vitro extraction and separation of copper ions from human blood samples based on amoxicillin/clavulanic acid by ultrasound assisteddispersive centrifuge liquid-liquid micro extraction
سال انتشار: 1399
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 63
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شناسه ملی سند علمی:
JR_AMECJ-3-1_005
تاریخ نمایه سازی: 14 آذر 1402
چکیده مقاله:
The low concentration of copper (Cu۲+) can be effected on the
central nervous system (CNS) and caused to multiple sclerosis
(MS). Although many antibiotics can treat the bacterial infections
but some of antibiotics decrease essential metal concentrations in
human body and must be controlled by determining. In this study,
in-vitro extraction of copper (Cu۲+) with amoxicillin/clavulanic
acid (AMOXC) has been studied due to interacting with metals.
By procedure, Cu۲+ ions were separated from blood samples by
ultrasound assisted-dispersive centrifuge liquid-liquid micro
extraction (USA-DC-LLME). The mixture of AMOXC (۰.۰۱
g), ionic liquid ([BMIM][PF۶]) and acetone injected to ۱۰ ml
of serum blood sample at human pH=۷.۲. After extraction, the
concentration of Cu۲+ ions was determined by flame atomic
absorption spectrometry (F-AAS). The LOD, enrichment factor
(EF), linear range (LR) and working range (WR) were obtained ۶
μg L-۱, ۹.۹۲, ۰.۰۱۸-۰.۵ mg L-۱ and ۰.۰۲-۲.۵۸ mg L-۱, respectively
(RSD<۱.۱%). The validation of technology was confirmed by
ICP and spiking samples.
کلیدواژه ها:
Copper ، Separation ، Human blood ، Amoxicillin/clavulanic acid ، Ultrasound assisted-dispersive centrifuge liquid-liquid micro extraction
نویسندگان
Jamileh Esmaeili
Ph.D Student in Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran , Iran- Department of Pharmacology, Tehran University of Medical Sciences
Samira Shirooie
Sciences Research center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
Azam Bakhtiarian
Department of Pharmacology Tehran University of medical Sciences