Simultaneously determination of copper and zinc in human serum and urine samples based on amoxicillin drug by dispersive ionic liquid- liquid microextraction coupled to flame atomic absorption spectrometry
سال انتشار: 1399
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 43
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شناسه ملی سند علمی:
JR_AMECJ-3-3_003
تاریخ نمایه سازی: 14 آذر 1402
چکیده مقاله:
In this work the effect of amoxicillin on copper and zinc (Cu and Zn)
deficiency was evaluated by determining of Cu and Zn concentration
in human serum and urine samples. By dispersive ionic liquid cloud
point extraction procedure (DIL-CPE), ۰.۰۳ g of pure amoxicillin
drug was added to mixture of ۰.۱ g of hydrophobic ionic liquid and
۰.۲ mL acetone which was injected to ۲ mL of serum or urine samples
which was diluted with DW up to ۱۰ mL. The cloudy solution was
shacked for ۷ min and Cu and Zn ions was extracted based on sulfur
group on amoxicillin ligand at pH of ۷ by DIL-CPE. Then, the solution
centrifuged and after back extraction with I mL of nitric acid (۰.۲ M),
the remained solution was determined by flame atomic absorption
spectrometry (F-AAS). The enrichment factor (EF), LOD and linear
range (LR) for copper and zinc was obtained (۹.۹۲; ۹.۸۱), (۲۸.۵ μg
L−۱; ۱۵.۲ μg L−۱) and (۱۰۰ -۵۰۵ μg L−۱; ۴۱- ۱۵۳ μg L−۱), respectively.
The results showed us, the concentration of the Cu and Zn ions can be
decreased by increasing amoxicillin drug dosage in human body. The
mean value for serum copper/zinc ratio was obtained ۱.۱۱ ± ۰.۲۸. The
DIL-LME method was validated by ICP-MS analysis and spike of
real samples for Zn and Cu ions in serum and urine samples.
کلیدواژه ها:
Amoxicillin drug ، Copper and zinc ، Serum and urine samples ، Dispersive ionic liquid cloud point extraction procedure ،
Flame atomic absorption spectrometry
نویسندگان
Kian Azami
Department of Pharmacology and Toxicology, Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box ۱۴۱۵۵-۶۴۵۱, Tehran, Iran
Seyed Mojtaba Mostafavi
Department of Chemistry, Iranian-Australian Community of Science, Hobart, Tasmania, Australia