Cobalt separation from water and food samples based on penicillamine ionic liquid and dispersive liquidliquid microextraction before determination by AT-FAAS

The cobalt compounds have adverse health effect on human and caused to damage of the DNA cells, neurological and endocrine systems. Therefore, the separation and determination of cobalt in water and food samples must be considered. In this research, the (۲S)-۲-amino-۳-methyl-۳-sulfanylbutanoic acid (penicillamine) as a chelating agent mixed with ionic liquid (OMIM PF۶) /acetone and used for extraction of cobalt from ۵۰ mL of water samples by ultraassisted dispersive liquid-liquid microextraction (USA-DLLME) at pH=۶. Based on procedure, the samples were shaked for ۵ min (۲۵oC) and after complexation of cobalt ions by thiol and amine group of penicillamine, the ionic liquid phase separated in the bottom of the conical tube by centrifuging for ۳.۰ min. The upper liquid phase was vacuumed by the auto-sampler and the Co۲+ ions back extracted from the ionic liquid/ penicillamine in acidic pH. Finally, the cobalt concentration in remained solution was determined by atom trap flame atomic absorption spectrometry (AT-FAAS). The main parameters such as the sample volume, the penicillamine amount, the ionic liquid amount and the shaking time were optimized. The linear range, the detection limit (LOD) and enrichment factor were obtained ۱.۵-۶۲ μg L-۱, ۰.۳۸ μg L-۱ and ۹۸.۵, respectively (r = ۰.۹۹۹۵, RSD%=۲.۲). The procedure was validated by ET-AAS analysis.