Investigating the Combinational Effect of Micellar Curcumin and Metformin on the Invasion of PC۳ Prostate Cancer Cell Line
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 74
فایل این مقاله در 12 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_TBSRJ-5-3_001
تاریخ نمایه سازی: 17 آذر 1402
چکیده مقاله:
Introduction: Adverse side effects, non-specific function, and increasing tolerability of tumor cells to chemotherapy demand therapeutic strategies with fewer side effects and better efficacy. Curcumin and metformin have been shown to have anticancer properties, according to emerging research. Therefore, micellar curcumin and metformin were combined and analyzed on cancer cell line PC۳.
Material and Methods: Cell viability after drug treatment, individually and in combination, was evaluated using an MTT assay. In order to determine the type of interaction between micellar curcumin and metformin, the median-effect method was employed. Subsequently, the expression of MMP-۲ and MMP-۹ of cells treated with single drugs and a synergistic combination of compounds was assessed using Gelatin Zymography test. Moreover, evaluating the number of invasive cells under treatment with drugs alone and in combination through matrigel invasion assay.
Results: The results demonstrated that the cell proliferation treated with micellar curcumin and metformin alone and in combination decreased significantly (P<۰.۰۵) during ۲۴ and ۴۸ h. Besides, co-treatment of compounds showed synergy within ۴۸ h. Although all the cells treated, whether with single drugs or the synergistic combination of both, showed a decline in expressing MMP-۲, MMP-۹, and the number of migrated cells, the maximum reduction was seen when micellar curcumin and metformin were combined.
Conclusion: This study showed that metformin and micellar curcumin together have a synergistic effect on cell proliferation inhibition in the PC۳ cell line. Metformin and micellar curcumin were more effective as a combination in decreasing the PC۳ cell line invasion by reducing MMP-۲ and MMP-۹.
کلیدواژه ها:
نویسندگان
Nazanin Ghahari
Department of Medical Biotechnology, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
Shadi Abkhiz
Department of Medical Biotechnology, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
Hamed Montazeri
Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Iran University of Medical Sciences, Tehran, Iran
Parastoo Tarighi
Department of Medical Biotechnology, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :