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مقالات فارسیISIکنفرانسهاژورنالها

Wound healing effects of dexpanthenol-loaded core/shell electrospun nanofibers: Implication of oxidative stress in wound healing

محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 27، شماره: 1
سال انتشار: 1403
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 72

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استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1846909

شناسه ملی سند علمی:

JR_IJBMS-27-1_013

تاریخ نمایه سازی: 18 آذر 1402

چکیده مقاله:

Objective(s): Knowing the detrimental role of oxidative stress in wound healing and the anti-oxidant properties of Dexpanthenol (Dex), we aimed to produce Dex-loaded electrospun core/shell nanofibers for wound healing study. The novelty was measuring oxidative stress in wounds to know how oxidative stress was affected by Dex-loaded fibers.Materials and Methods: TPVA solution containing Dex ۶% (w/v) (core) and PVA/chitosan solution (shell) were coaxially electrospun with variable injection rates of the shell solution. Fibers were then tested for physicochemical properties, drug release profile, and effects on wound healing. Levels of tissue lipid peroxidation and superoxide dismutase activity were measured.Results: Fibers produced at shell injection rate of ۰.۳ ml/hr (F۳ fibers) showed core/shell structure with an average diameter of ۲۵۲ nm, high hydrophilicity (swelling: ۱۵۷% at equilibrium), and low weight loss (۱۳.۶%). Dex release from F۳ fibers seemed to be ruled by the Fickian mechanism based on the Korsmeyer-Peppas model (R۲ = ۰.۹۴, n = ۰.۳۷). Dex-loaded F۳ fibers promoted fibroblast viability (۱۲۸.۴%) significantly on day ۵ and also accelerated wound healing compared to the neat F۳ fibers at macroscopic and microscopic levels on day ۱۴ post-wounding. The important finding was a significant decrease in malondialdehyde (۰.۳۹ nmol/ mg protein) level and an increase in superoxide dismutase (۵.۲۹ unit/mg protein) activity in Dex-loaded F۳ fiber-treated wound tissues. Conclusion: Dex-loaded core/shell fibers provided nano-scale scaffolds with sustained release profile that significantly lowered tissue oxidative stress. This finding pointed to the importance of lowering oxidative stress to achieve proper wound healing.

کلیدواژه ها:

Core/shell nanofiber ، Dexpanthenol ، Fibroblast ، Mouse ، Oxidative stress ، Wound healing

نویسندگان

Seyede Sahar Hashemi

Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran

Mahmoud Najari

Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran

Milad Parvin

Department of Oral & Maxillofacial Surgery, School of Dentistry, Bushehr University of Medical Sciences, Bushehr, Iran

Mohammad Mehdi Kalani

Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran

Majid Assadi

Nuclear Medicine and Molecular Imaging Research Center, Bushehr University of Medical Sciences, Bushehr, Iran

Ramin Seyedian

Department of Pharmacology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran

Sasan Zaeri

Department of Pharmacology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran

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