A Plastic-Contaminated Soil Gene Catalog Constructed byMetagenomic Approaches

Plastics are widely used worldwide for numerous goals, and polluted marine and terrestrial environment by plas tics has become an intense ecological obstacle. Plastic recycling is very tardy, and polymer residues harm differentenvironments, such as humans and animals. The roles of plastic-exposed environment microbiome are conclu sive for identifying microbes and enzymes to advance plastics degradation. Combining computational methodsand metagenomic approaches is crucial to increase the current plastic degradation methods. Gene, protein andmetagenome-assembled genomes catalogs simplify taxonomical and functional profiling of microbiome samples .Based on our knowledge, no integrated gene catalogs from plastic-contaminated environments exist. In this study,۶۶ whole metagenome samples (including soil , , leachate, sludge , and plastic fabrics samples ) were combinedfrom four exiting metagenome data with our new sample. A mean library fragment size of ۳۶۵ Gb of high-qualitydata was obtained; this data size is excellent than all of the used plastic-contaminated samples. A plastic contaminated soil gene catalog (PCSGC) and a plastic-contaminated soil protein catalog (PCSPC) were generatedby expanding a computational workflow composed of quality control of reads, trimming, assembly, and binning ofcontigs. These catalogs contain ۵۳.۳ million nonredundant genes and proteins. Also, glycoside hydrolases (GH)and Carbohydrate-Binding modules (CBM) were the most frequent carbohydrate-active enzymes (CAZymes) thatwere generated using dbCAN۲ software. dbCAN۲ resulted in the ۲۰۵,۰۶۶ CAZyme-encoding genes in the PCSGC,which belong to ۵۱ CAZyme subclasses. Our workflow and generated catalog of plastic-contaminated samples canhelp researchers to expand their knowledge of plastic-associated genes and proteins with minimal time and cost.