Investigating the Relationship Between A/T ۲۵۱ Polymorphism of IL-۸ Gene and Cancer Recurrence After Lumpectomy

Introduction: This research investigates the relationship between A/T۲۵۱ polymorphism of IL-۸ gene (as a genetic marker) with cancer recurrence after lumpectomy and instead of PCR-RFLP method which most common and expensive method for detecting polymorphisms is the more economical technique of PCR Tetra Arms.Methodology: After DNA extraction, a nano drop device was used to evaluate the quantity and quality of DNA and to know its concentration and degree of purity. ۱ microliter of the extracted DNA was placed in the device and its optical absorption was read at different wavelengths. For the correct performance of the method and in order to determine the sequence and confirm the PCR product, several samples of dominant homozygotes, recessive homozygotes and heterozygotes were selected and purified by Cena clone kit.Results: Heterozygous individuals were identified with bp۱۶۹ and bp۲۲۸ long fragments along with bp۴۳۹ control band. If mutant homozygous individuals with A allele showed bp ۲۲۸ fragment and wild homozygous individuals with T allele showed bp ۱۶۹ fragment on ۲% electrophoresis gel.Conclusion: Several cytokines, including IL-۸, play an important role in causing laminogenic inflammation in tissue and as a result, causing and aggravating cancers. These studies have studied the role of IL-۸ gene polymorphisms and its relationship with types of cancer in different populations. Therefore, this study was conducted with the aim of investigating the ۲۵۱ A/T polymorphism of the IL-۸ gene in the population of Iranian women with breast cancer and the results of this study showed.