Capacitive Hyperthermia as an Alternative to Brachytherapy in Treatment of Human Prostate Cancer Cell

Introduction: The aim of this study was the evaluation of induced DNA damages of human prostate cancer cells, DU-۱۴۵, treated with a combination of radiofrequency capacitive hyperthermia (HT) and teletherapy (EBRT) compared to a combination of teletherapy with high dose rate brachytherapy (BR).   Materials and Methods: DU-۱۴۵ cells were cultured as spheroids in ۳۰۰-micron diameter. Then the following treatments were conducted: a) EBRT at doses of either ۲Gy or ۴Gy of photon ۱۵MV, b) HT for ۳۰, ۶۰, and ۹۰ minutes’ duration at ۴۳°C from a ۱۳.۵۶MHz radiofrequency capacitive heating device (Celsius TCS), c) BR with Ir-۱۹۲ seed at doses of either ۲Gy or ۵.۵Gy, d) The mentioned HT followed by EBRT (HT+EBRT) and e) EBRT followed by BR (EBRT+BR). Alkaline comet assay was performed to measure tail moment. Results: The induced DNA damages of DU-۱۴۵ cells treated by adding HT to EBRT compared with EBRT alone, showed a significant enhancement; ۳.۲۸ and ۵.۱۴ times respectively for ۳۰ and ۶۰ minutes HT. By plotting dose-response curves, we could find a range of doses, which create radiobiological iso-effect in HT+EBRT and EBRT+BR treatments. Conclusion: This study suggests that about DNA damages of DU-۱۴۵ cells, HT+EBRT could partly be considered as an alternative to EBRT+BR.