The effect of recombinant nanomedicine of ۵-fluorouracil and sodium butyrate on microRNA let۷ on mcf۷ breast cancer cell line

The treatment for certain cancers requires the use of recombinant drugs. The majority of patients eventually acquire treatment resistance, and new mutations render old treatments ineffective, as a result of our glacial pace in designing and executing clinical trials for effective medications and the tsunami-grow like development of cancer.Purpose: The aim of this study was to enhance one of the drugs that caused significant resistance in individuals with breast cancer and combine it with another component in ordering to increase its efficacy.Method: The effects of sodium butyrate and ۵-fluorouracil nanomedicine on the mcf۷ cell line were investigated with a focus on Mir Let۷ using MTT assay and real-time PCR. The cells were exposed to nanomedicine using the MTT method for ۲۴, ۴۸, and ۷۲ hours, and then the Real-time PCR method was used to assess the expression levelDiscussion:Previous research has identified Mir-let۷ as one of the breast cancer indicators. This microRNA functions as a tumor suppressor in the cell, inhibiting tumor cell migration and proliferation as well as decreasing the expression of Mir let۷ in these cells.