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مقالات فارسیISIکنفرانسهاژورنالها

Study on Propagation and Adaptation of EDS-۷۶ Avian Adenovirus in Duck and SPF Primary Embryonic Chicken Cell Culture Comparison to Duck and SPF Embryonated Chicken Eggs

محل انتشار: مجله آرشیو رازی، دوره: 78، شماره: 3
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 60

فایل این مقاله در 11 صفحه با فرمت PDF قابل دریافت می باشد

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لینک ثابت به این مقاله:
https://civilica.com/doc/1867597

شناسه ملی سند علمی:

JR_ARCHRAZI-78-3_038

تاریخ نمایه سازی: 6 دی 1402

چکیده مقاله:

Egg drop syndrome (EDS) is a major viral infectious poultry disease with severe economic losses in laying hens. The disease is caused by an adenovirus and can be transmitted horizontally and vertically. This study investigated the EDS virus (EDSV) infection in duck embryo fibroblasts (DEF), specific pathogen-free (SPF) embryo fibroblasts, and SPF egg embryos using different methods. The results were compared to the virus culture in duck and SPF chicken eggs. Duck and chicken fibroblast cells were used as the primary cell culture in Dulbecco’s Modified Eagle Medium, and the low-pathogenic duck adenovirus was used to infect the ducks and SPF fibroblasts primary cell cultures, as well as the duck and SPF eggs. The titer of the virus was measured by hemagglutination assay, ECID۵۰, plaque-forming unit, and TCID۵۰ methods. The results revealed that EDSV could proliferate in the chorioallantoic membrane of DEF cells and duck eggs, compared to the chorioallantoic membrane of chicken embryo fibroblasts (CEF) and SPF chicken eggs. The findings showed that duck egg embryos and primary DEF cell lines are more appropriate for EDSV replication, compared to CEF and SPF chicken eggs. This suggests that the use of DEF culture for producing avian adenovirus EDS-۷۶ is a suitable alternative for the embryonic egg culture.

کلیدواژه ها:

EDS virus ، Adenovirus ، duck embryo fibroblast ، Cell culture

نویسندگان

A. A Razmarai Iranagh

Young Researchers and Elite Club, Islamic Azad University, Shabestar, Shabestar, Iran

N. Razmaraii

Razi Vaccine and Serum Research Institute Northwest Branch, Agricultural Research, Education and Extension Organization (AREEO), Marand, Iran

R Aghaei

Department of Veterinary Medicine, Islamic Azad University, Shabestar Branch, Islamic Azad University, Shabestar, Iran

J Shayegh

Department of Veterinary Medicine, Islamic Azad University, Shabestar Branch, Islamic Azad University, Shabestar, Iran

M Mousaviyan

Department of Cellular and Molecular Biotechnology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

A Ameghi Roudsary

Department of Cellular and Molecular Biotechnology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

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