Effects of Intracerebroventricular Injection of the Steroidal and Non-Steroidal Anti-Inflammatory Drugs on the Seizures during the Estrous Cycle in Rat

Because of the mutual relationship between neural inflammation and seizure, this study aimed to determine the effects of intracerebroventricular (ICV) injection of the steroidal and non-steroidal anti-inflammatory drugs on pentylenetetrazol (PTZ)-induced seizures during the estrous cycle in rats. A total of ۱۰۵ adult female Wistar rats were selected and divided into seven groups, including the control (saline), ketorolac tris salt (۷.۵, ۱۵, and ۳۰ µg), and methylprednisolone acetate (۰.۱۵, ۰.۳, and ۰.۶ µg), each with four subgroups (proestrus, estrus, metestrus, and diestrus) and three replicates (n=۵). After a week of acclimatization, the estrous phase determination and synchronization were performed. Acute epilepsy was inspired by the intraperitoneal injection of ۸۰ mg/kg of PTZ ۳۰ min after the ICV injection of ketorolac and methylprednisolone acetate. The initiation time of myoclonic seizures (ITMS), the initiation time of tonic-clonic seizures (ITTS), seizure duration (SD), and mortality rate (MR) were measured for ۳۰ min. Data were shown as mean±SD and analyzed using One-way ANOVA followed by Tukey–Kramer multiple comparison post hoc test (P<۰.۰۵). According to the results, ketorolac (۱۵ and ۳۰ µg) and methylprednisolone acetate (۰.۳ and ۰.۶ µg) significantly increased the ITTS and ITMS but decreased SD during the estrous cycle, compared to the control (P<۰.۰۵). Moreover, MR and SD were significantly decreased by ketorolac (۷.۵, ۱۵, and ۳۰ µg) and methylprednisolone (۰.۳ and ۰.۶ µg), compared to the control during the estrous cycle (P<۰.۰۵). Therefore, it seems that both ketorolac and methylprednisolone possess dose-dependent anticonvulsant effects that may decrease neural inflammation.