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مقالات فارسیISIکنفرانسهاژورنالها

Monitoring of Newcastle Disease Virus Vaccine Strain Replication in Embryonated Chicken Eggs by Reverse Transcription-Polymerase Chain Reaction

محل انتشار: مجله آرشیو رازی، دوره: 78، شماره: 2
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 58

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https://civilica.com/doc/1867636

شناسه ملی سند علمی:

JR_ARCHRAZI-78-2_033

تاریخ نمایه سازی: 6 دی 1402

چکیده مقاله:

The knowledge of virus and replication kinetics plays a key role in developing a vaccine. This study aimed to monitor the replication process and determine the best harvesting time of the Newcastle disease virus (NDV) V۴ vaccine strain in the allantoic fluids of specific pathogen-free (SPF)-embryonated chicken eggs (ECEs) by reverse transcription-polymerase chain reaction (RT-PCR), hemagglutination (HA), and egg infective dose ۵۰% (EID۵۰) tests. For this purpose, the V۴ vaccine strain of the virus was intra-allantoically inoculated into ۹۶ ۱۰-day-old SPF-ECEs at the rate of ۰.۱ mL/ECE. The allantoic fluids of the inoculated eggs were collected from six eggs at six-hour intervals up to ۹۶ hours post-infection (hpi). The harvested suspensions were confirmed to contain the NDV by the mentioned serologic and molecular techniques. Based on the results, the virus was first detected at ۳۶ hpi in ECEs by RT-PCR. The peak of HA and EID۵۰ titers in allantoic fluids started at ۴۲ hpi, and the titers were at the highest level until the end of the experiment. The results indicated that the best virus harvesting time for the NDV V۴ vaccine strain in ECEs is between ۴۲-۶۰ hpi. These findings pave the way for adequate and enhanced production rate, immunogenicity, and cost-related parameters in the V۴ Newcastle vaccine development.

کلیدواژه ها:

Embryonated chicken egg ، Newcastle disease virus ، Reverse transcription-polymerase chain reaction ، vaccine

نویسندگان

R Ghadimipour

Department of Research and Development, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Marand, Iran

M Taghizadeh

Department of Research and Development, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

M Bashashati

Department of Avian Disease Research and Diagnostic, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

M. M Ebrahimi

Department of Poultry Research and Vaccine Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

A Samadi

Department of Research and Development, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Marand, Iran

S Mohammadzadeh

Department of Biology, Faculty of Basic Sciences, Marand Branch of Islamic Azad University, Marand, Iran

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