Evaluation of the Efficacy of Sodium Chloride Nanoparticles on the Vitality of Leishmania Major (in vitro)
محل انتشار: مجله آرشیو رازی، دوره: 78، شماره: 2
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 33
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شناسه ملی سند علمی:
JR_ARCHRAZI-78-2_015
تاریخ نمایه سازی: 6 دی 1402
چکیده مقاله:
Leishmaniasis is one of the most important zoonotic diseases transmitted to humans by sand flies (Phlebotomus spp). Leishmania major promastigote causes Cutaneous Leishmaniasis in humans. The study aimed to investigate the effectiveness of Sodium Chloride nanoparticles (NaCl NPs) on the vitality of Leishmania major promastigote compared with the standard dose of Pentostam under laboratory conditions. Various concentrations of ۲, ۴, ۶, and ۸ μg/ml of the NaCl NPs were prepared. These concentrations were tested in vitro on L. major growth by the culture of the parasite in the cell culture microplate. After the fourth day, a different concentration of NaCl NPs was added with three replicates for each concentration. Later, the numbers of promastigotes were counted daily using a Haemocytometer stained by Trypan blue solution stain duration of the study, which continued for four days. The results showed that the Growth Index (GI) rate of L. major promastigote was decreased with increasing NaCl NPs concentration. The Growth Index rates were ۱.۳۲×۱۰۶, ۱.۳۱×۱۰۶, ۰.۹۵×۱۰۶, and ۰.۷۸×۱۰۶for the mentioned concentrations. These values were compared with the rate of the Pentostam group and control group, which were ۱.۰۹×۱۰۶ and ۳.۴۳×۱۰۶, respectively. The results revealed that the highest inhibition percentage was ۹۲% for ۸ μg/ml NaCl NPs after ۹۶ hours, Pentostam group and control group, which were %۸۶ and %۰.۰۰ for inhibition promastigote, respectively in the same period. The statistical analysis revealed a significant difference among concentrations at P≤۰.۰۵ compared with the Pentostam and control groups. The current study concluded that the NaCl NPs have an excellent biological effect in inhibiting L. major promastigote growth in vitro. These promising results paved the way for employing NaCl NPs to treat human cutaneous leishmaniasis.
کلیدواژه ها:
نویسندگان
I Abbas Marhoon
Department of Biology, College of Education, University of Al-Qadisiyah, Al-Qadisiyah, Iraq
M Majid Al-Musawi
Department of Biology, Faculty of Education for Girls, University of Kufa, Kufa, Iraq
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