Secretory Excretory and Somatic Immunogenic Antigens Profiles of Adult Fasciola spp.
محل انتشار: مجله آرشیو رازی، دوره: 78، شماره: 1
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 42
فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_ARCHRAZI-78-1_052
تاریخ نمایه سازی: 6 دی 1402
چکیده مقاله:
Fascioliasis is a common human-animal disease that is reported in most parts of the world. Fascioliasis is also prevalent in different provinces of Iran. Since it has done no study on the excretory/secretory and somatic immunogenic antigens profiles of adult Fasciola in Iran, the present study was performed on the Fasciola spp. collected from Mazandaran province. For this purpose, the Fasciola worm was isolated from the liver of infected sheep, then its excretory/secretory and somatic antigens were prepared from adult worms. The protein of the samples was measured by the Lowry method. Then, somatic and secretory excretions were examined by SDS-PAGE and the protein profile of the two substances was determined. To evaluate the immunogenicity, the somatic and secretory excretions antigens of Fasciola spp. were injected into white rabbits and after boosting, the blood serum of the rabbits was collected and then Western blotting was performed on them and the results were evaluated. According to the results of Western blotting, ۱۱ somatic antigen bands with a molecular weight of ۱۴۹, ۱۲۲, ۹۹, ۸۵, ۷۵, ۶۵, ۵۰, ۴۶, ۴۰, ۳۷, ۳۰ kDa and ۱۲ protein bands of excretory/secretory antigens with molecular weights of ۱۰۰, ۸۲, ۷۵, ۷۰, ۵۸, ۵۵, ۴۷, ۴۰, ۳۸, ۳۷, ۳۰,۲۵ kDa were observed in adult Fasciola spp. that immunogenic, which appear to have a protective effect or can be used to prepare a diagnostic kit.
کلیدواژه ها:
نویسندگان
A Dezhabad
Chalous Branch, Islamic Azad University, Chalous, Iran
A Dalimi
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
N Hoghooghi Rad
Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
R Madani
Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :