Molecular-Genotyping Detection of Entamoeba histolytica in Diarrheic Patients
محل انتشار: مجله آرشیو رازی، دوره: 78، شماره: 1
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 34
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شناسه ملی سند علمی:
JR_ARCHRAZI-78-1_040
تاریخ نمایه سازی: 6 دی 1402
چکیده مقاله:
Amebiasis is caused by Entamoeba histolytica, a protozoan that is found worldwide. The degree of pathogenesis of clinical isolates varies greatly. This study was aimed to molecular identification of E. histolytica in children using the nested polymerase chain reaction (nPCR), and then, a genotyping of positive E. histolytica isolates using the quantitative PCR (qPCR) assay through targeting serine-rich E. histolytica protein (SREHP) gene. For this purpose, a total of ۵۰ bloody diarrheic stool samples were collected from the children attended to the Al-Zahraa’ Teaching Hospital and Alkut Hospital for Gynecology, Obstetric and Pediatrics (Alkut, Wasit, Iraq) were subjected to the present study from September to December ۲۰۲۱. Firstly, the extracted DNAs that amplified using specific primers through targeting ۱۸S rRNA gene and tested using nPCR assay were revealed an overall ۴۸% (۲۴/۵۰) positive samples for E. histolytica. For genotyping, our results were detected an existence of four different genotypes (I, II, III and IV) with a significant prevalence of Genotype-II (۵۴.۱۷%) when compared to Genotype-I (۲۰.۸۳%), Genotype-III (۱۲.۵%) and Genotype- IV (۱۲.۵%). In addition, results of melting temperature of targeted genotypes were ۸۴ºC, ۸۳ - ۸۳.۵ºC, ۸۲.۵ºC and ۸۱ºC for Genotype-I, II, III and IV, respectively. In conclusion, molecular amplification of ۱۸S rRNA gene was revealed the large prevalence of E. histolytica among bloody diarrheic children of study areas; while, amplification of SREHP gene was reflected the widespread phenotypic variation of the Genotype-II suggesting the high ability of this genotype to spread infection in children. In different endemic areas as Iraq, the utilization of high-resolution genotyping methods showed the extremely polymorphic genetic structure of this parasite.
کلیدواژه ها:
نویسندگان
D Kareem Kadhim
Department of Anatomy and Biology, College of Medicine, University of Wasit, Wasit, Iraq
B Abdulsalam Hraija
Department of Microbiology, College of Medicine, University of Wasit, Wasit, Iraq
G Aqeele
Department of Microbiology, College of Medicine, University of Wasit, Wasit, Iraq
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