Effect of Ultraviolet Light on the Expression of icaD Gene in Staphylococcus aureus Local Isolates in Iraq
محل انتشار: مجله آرشیو رازی، دوره: 76، شماره: 5
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 55
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شناسه ملی سند علمی:
JR_ARCHRAZI-76-5_008
تاریخ نمایه سازی: 6 دی 1402
چکیده مقاله:
The current study was designed to reveal the impact of ultraviolet (UV) light on polysaccharide intercellular adhesion of the icaD gene expression and its relation to biofilm as virulence markers in Staphylococcus aureus. In total, ۳۷ isolates of S. aureus were gathered from various bacterial infection sources, and ۲۶ S. aureus isolates were definite to icaD gene using the Polymerase Chain Reaction (PCR) technique. Prior to and after exposure to UV light, RNAs from the isolates were extracted which had strong biofilm formation by crystal violet staining assay, and they were then exposed to quantitative real-time PCR (qPCR). In vitro, the survival of isolates was evaluated after the exposition to several periods of the UV light power via counting the number of colonies. The results showed that the exposure to the UV light at ۳۲۰ nm for ۲, ۴, ۶, ۸, and ۱۰ min had inhibitory effects on the bacterial growth. The sub-lethal exposure time was ۸ min. The rapid reduction of bacterial survival rates was associated with the increment irradiation time of UV power from ۵ to ۱.۲۴ log۱۰ CFU/ml. In general, down regulation of the icaD gene expression was decreased upon exposure to the UV light which was used as a physical agent for controlling the bacterial infection and biofilm formation.
کلیدواژه ها:
نویسندگان
S Hamel Mohaisen
Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, University of Baghdad, Baghdad, Iraq
M Hussain Ali
Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, University of Baghdad, Baghdad, Iraq
Z Hashem Shehab
Biology Department, College of Science for Women, University of Baghdad, Baghdad, Iraq
A. W Al-Mayyahi
Employee in the Ministry of Health, Iraq
A. A Abdulhassan
Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, University of Baghdad, Baghdad, Iraq
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