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مقالات فارسیISIکنفرانسهاژورنالها

Pharmacological Effect of Caffeine on Drosophila melanogaster: A Proof-of-Concept in vivo Study for Nootropic Investigation

محل انتشار: مجله آرشیو رازی، دوره: 76، شماره: 6
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 46

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لینک ثابت به این مقاله:
https://civilica.com/doc/1868085

شناسه ملی سند علمی:

JR_ARCHRAZI-76-6_008

تاریخ نمایه سازی: 6 دی 1402

چکیده مقاله:

A comprehensive investigation into drug candidates with nootropic activity using a proper and high throughput yet economical model organism is an important issue to consider. This proof-of-concept study was carried out to determine whether Drosophila melanogaster can be used as an in vivo screening platform to assess the nootropic activity of certain candidates for the treatment of neurodegenerative diseases. To test this, caffeine was used as a nootropic compound and a Drosophila mutant line lacking PGRP-LB with hyperactivation of NF-κB leading to early death with neurodegenerative phenotype was used as a model organism. Caffeine was orally administered via food to the PGRP-LB mutant of D. melanogaster at different concentrations (۰.۴ mM, ۰.۰۸ mM, ۰.۰۱۶ mM) prior to phenotypical observations of the survival and locomotor activity, as well as gene expression analysis, to assess the expression level of sod۱, sod۲, and cat genes. The results pointed out that the lifespan of D. melanogaster treated with ۰.۰۱۶ mM caffeine was dramatically increased; nonetheless, no changes were observed in the locomotor activity. Phenotypical analysis using a T-maze vial test demonstrated a good cognitive improvement in response to caffeine administration. Molecular analysis revealed that caffeine at a concentration of ۰,۰۱۶ mM induced the expression of the endogenous antioxidant genes sod۱ and cat, but not sod۲, signifying that the increased lifespan may be associated with a marked improvement in cytoplasmic antioxidant function. In general, the findings of the present study are in line with those previously observed in the mammalian model organism. Therefore, it can be concluded that D. melanogaster can be used as a model organism in preliminary investigation and screening of nootropic candidates prior to further testing in its mammalian counterparts.

کلیدواژه ها:

Nootropic ، caffeine ، neurodegenerative disorders ، Fruit fly ، Cognitive function

نویسندگان

A Asbah

Faculty of Pharmacy, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia

U Ummussaadah

Faculty of Pharmacy, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia

N Parenden

Faculty of Pharmacy, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia

A. S. W Putri

Faculty of Medicine, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia

R. A Rosa

Faculty of Pharmacy, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia

N. R Rumata

Sekolah Tinggi Farmasi Makassar, Makassar, South Sulawesi, Indonesia

T. B Emran

Department of Pharmacy, BGC Trust University, Chittagong-۴۳۸۱, Bangladesh

K Dhama

Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-۲۴۳۱۲۲, Uttar Pradesh, India

F Nainu

Faculty of Pharmacy, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia

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