Genetic Sequence of Coronavirus Strains Isolated from Iraqi Patients and their Relationship with some Liver Enzymes and Interleukins
محل انتشار: مجله آرشیو رازی، دوره: 77، شماره: 2
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 49
فایل این مقاله در 11 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_ARCHRAZI-77-2_037
تاریخ نمایه سازی: 6 دی 1402
چکیده مقاله:
The severe acute respiratory syndrome coronavirus ۲ (SARS-CoV-۲) which is a positive-sense single-stranded RNA virus from the genus Betacoronavirus causes COVID-۱۹ (coronavirus disease ۲۰۱۹). According to daily reports issued by the Iraqi Ministry of Health, the SARS-COV-۲ was firstly detected in Al-Najaf city in February ۲۰۲۰ and identified in the Central Public Health Laboratory (CPHL) in Baghdad, Iraq. The outcomes of this study were based on ۱۰۰ nasopharyngeal swaps and venous blood samples from hospitalized patients in Al-Kindy and CPHL. Patients were assigned to five groups (Asymptomatic, Mild, Moderate, Severe, and Deceased) based on disease severity as indicated by World Health Organization (WHO). The positive samples were identified by real-time quantitative polymerase chain reaction (RT-PCR) and subjected to some liver enzyme assays and interleukins measurements, and the correlation with the genetic sequence was determined by Illumina Miseq technology. Liver enzymes levels of Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) showed statistically significant differences, especially between the deceased groups. Interleukins (IL-۱۰, IL-۱۸, and TNF- α) significantly differed among groups. This study revealed that three isolates belonging to the original strain isolated from Wuhan (A۱۹) and characterized by their virulence caused severe symptoms and led to admission to isolation hospitals and intensive care units, and the last two isolates of (UK alpha V۱) appeared in Iraq in early ۲۰۲۱. These strains which were less virulent than the Wuhan strain spread faster and appear in moderate and asymptomatic patients.
کلیدواژه ها:
نویسندگان
R. A Salih
Central Public Health Laboratory, Ministry of Health, Baghdad, Iraq
N. S Mohamed
Pharmacy College, Al- Nahrain University, Baghdad, Iraq
A. A Taha
Department of Applied Science, University of Technology, Baghdad, Iraq
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :