Assessment of Contagious Ecthyma Virus in Camels of Wasit Province, Iraq

سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 40

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شناسه ملی سند علمی:

JR_ARCHRAZI-77-2_006

تاریخ نمایه سازی: 6 دی 1402

چکیده مقاله:

Camel contagious ecthyma (CCE) is an infectious disease caused by the Paravox virus (PPV) of the family Poxviridae. Due to the importance of the camel breeding industry in tropical and subtropical regions, the present study aimed to isolate the causative agent of camel contagious ecthyma (CCE) using cell culture and molecular confirmation of virus isolate. A total of ۲۱۰ camels aged ۶ months to ۴ years were selected from different districts in Wasit province (Iraq) from August ۲۰۱۷ to April ۲۰۱۹. These animals, which included ۱۱۷ females and ۹۳ males, displayed signs of papules, blisters, pustules, and scabs on the skin. To isolate the CCE virus, primary and secondary cell cultivation was performed using the lamb testis (LT) cells. The findings pointed out that there were cytopathic effects during the second passage of the virus, characterized by rounding and cells aggregation after ۷۲ h. Furthermore, there were dramatic changes, including sloughing off and detachment from the surface of the monolayer, in monolayer cells after ۴۸-۷۲ h. The titration values of the isolated Orf virus in LT cells were obtained at ۱۰-۵ TCI۵۰ /۰.۰۵ and ۱۰-۶ TCID ۵۰ / ۰.۰۵ ml in the third and fourth passages, respectively. As expected, the B۲L gene of affected camels was amplified from a skin biopsy DNA sample to produce nearly ۵۹۴ base pairs. In conclusion, the results of the current study focused on epidemiological and virological characteristics of CCE in Wasit province; moreover, the virus was confirmed by a specific gene called the B۲L gene.

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نویسندگان

H. A. M Al-Bayati

Department of Microbiology, College of Medicine, Wasit University, Wasit, Iraq

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