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مقالات فارسیISIکنفرانسهاژورنالها

Preparation and Characterization of Nanoliposome Containing Isolated VP۱ Protein of Foot and Mouth Disease Virus as a Model of Vaccine

محل انتشار: مجله آرشیو رازی، دوره: 77، شماره: 1
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 59

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https://civilica.com/doc/1868198

شناسه ملی سند علمی:

JR_ARCHRAZI-77-1_005

تاریخ نمایه سازی: 6 دی 1402

چکیده مقاله:

Foot-and-mouth disease (FMD) is an acute and highly contagious disease in livestock, such as cattle, sheep, and pigs, leading to a lot of economic losses. The current FMD vaccines formulated by inactivated whole-virus and adjuvant successfully reduce disease outbreaks in many regions of the world. Immunological studies on FMD viruses revealed that the dominant epitope in arising neutral antibody response is amino acid residues constructing the G-H loop, constituting a surface loop of the structural protein, termed VP۱. Liposomes as one of the most well-known vehicles are considered an important carrier in vaccine development, and their function is used to encapsulate purified VP۱ protein based on their size, charge, and lipid content. Accordingly, the VP۱ protein was isolated from the FMD virus. This study aimed to compare four methods of VP۱ protein encapsulation in the liposome and the extruding effect, as follows: ۱) VP۱ protein was dissolved in dimethyl sulfoxide and added to the lipid film hydrated by ethanol, ۲) the lipid film was hydrated by VP۱ protein with ۷M urea, ۳) the lipid film was hydrated by VP۱ protein and freeze-thawed, and ۴) the lipid film was hydrated by VP۱ protein. The highest encapsulation efficiency was ۹۱% in the second method which purified protein-containing urea. The VP۱ protein in the prepared liposome (۱, ۲-dimyristoyl-sn-glycero-۳-phosphocholine: ۱, ۲-dimyristoyl-sn-glycero-۳-phosphocholine: cholesterol) released more than ۹۰% of protein content after ۲۴۰ h.

کلیدواژه ها:

Encapsulation ، FMDV ، liposome ، PURIFICATION ، VP۱ protein

نویسندگان

M Kazemi

Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran

R Madani

Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

M. R Aghamaali

Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran

T Emami

Department of Proteomics and Biochemistry, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

F Golchinfar

Department of Proteomics and Biochemistry, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

L Heshmati

Department of Medical Nanotechnology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran

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