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مقالات فارسیISIکنفرانسهاژورنالها

Isolation, Characterization, and Efficacy of Three Lytic Phages Infecting Multidrug-Resistant Salmonella Serovars from Poultry Farms in Egypt

محل انتشار: مجله آرشیو رازی، دوره: 76، شماره: 3
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 45

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https://civilica.com/doc/1868289

شناسه ملی سند علمی:

JR_ARCHRAZI-76-3_011

تاریخ نمایه سازی: 6 دی 1402

چکیده مقاله:

Multidrug-resistant (MDR) Salmonella serovars are considered a significant threat to veterinary and public health. Developing new antimicrobial compounds that can treat the infection caused by these notorious pathogens is a big challenge. Bacteriophages can be adsorbed on and inhibit the growth of bacteria, providing optimal and promising alternatives to chemical antimicrobial compounds against foodborne pathogens due to their abundance in nature and high host specificity. The objective of the current study was to isolate and characterize new phages from poultry farms and sewage and to evaluate their efficacy against S. Enteritidis isolates. The study reports three lytic phages designated as ϕSET۱, ϕSET۲, and ϕSET۳ isolated from poultry carcasses and sewage samples in Qalubiya governorate Egypt. The effectiveness of phages was evaluated against multidrug-resistant S. Enteritidis strains. Electron microscopy showed that these phages belong to the Siphoviridae family. Phages were tested against ۱۳ bacterial strains to determine their host range. They could infect four S. Enteritidis and one S. Typhimurium; however, they did not infect other tested bacterial species, indicating their narrow infectivity. The bacteriophage's single-step growth curves revealed a latent period of ۲۰ min for ϕSET۱ and ۳۰ min for ϕSET۲ and ϕSET۳. The isolated Salmonella phages prevented the growth of S. Enteritidis for up to ۱۸ hrs. The findings revealed that Salmonella phages could be used as alternative natural antibacterial compounds to combat infection with MDR S. Enteritidis in the poultry industry and represent a step forward to using large panels of phages for eliminating Salmonella from the food chain.

کلیدواژه ها:

S. enteritidis ، Bacteriophage ، Siphoviridae

نویسندگان

H Sobhy

Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh PO Box ۱۳۷۳۶, Egypt

E. Soliman

Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh PO Box ۱۳۷۳۶, Egypt

A. A Abd El-Tawab

Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh PO Box ۱۳۷۳۶, Egypt

F. Elhofy

Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh PO Box ۱۳۷۳۶, Egypt

A Askora

Department of Microbiology and Botany, Faculty of Science, Zagazig University, ۴۴۵۱۹, Zagazig, Egypt

E. El-Nahas

Department of Virology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh PO Box ۱۳۷۳۶, Egypt

G Wareth

Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh PO Box ۱۳۷۳۶, Egypt

W Ahmed

Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh PO Box ۱۳۷۳۶, Egypt

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