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مقالات فارسیISIکنفرانسهاژورنالها

Development of New Modified Simple Polymerase Chain Reaction and Real-time Polymerase Chain Reaction for the Identification of Iranian Brucella abortus Strains

محل انتشار: مجله آرشیو رازی، دوره: 74، شماره: 3
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 56

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لینک ثابت به این مقاله:
https://civilica.com/doc/1868638

شناسه ملی سند علمی:

JR_ARCHRAZI-74-3_002

تاریخ نمایه سازی: 6 دی 1402

چکیده مقاله:

Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents. In this regard, the identification of Brucella can be a time-consuming and labor-intensive process posing a real risk of laboratory-acquired infection to the laboratory staff. This study aimed to present a novel conventional and real-time polymerase chain reaction (PCR) assay for the identification of Brucella abortus strains. Regarding this, two primers (bru ab۲) were designed based on the unique loci encoding autotransporter-associated beta strand repeat-containing protein (ID:YP۰۰۱۱۳۷۶۰). A total of ۵۶ Brucella strains (e.g., reference, vaccinal, and field isolates) and Yersinia enterocolitica, as a non-Brucella isolate, were evaluated in conventional and real-time PCR systems. The results of the study indicated that ۰.۴ ng and ۴۰۰ FG of genomic DNA of B. abortus strains can be detected by conventional and real-time PCR, respectively. The primers, bru ab۲, were suitable for both PCR methods. Both methods were specific for the detection of all strains of the bacterium; however, real-time PCR assay was ۱۰۰۰-fold more sensitive than the conventional PCR method. Therefore, this new detection system could be a suitable selective modified method for the accurate identification of all B. abortus strains.

کلیدواژه ها:

Brucella abortus ، identification ، PCR ، Real-time PCR

نویسندگان

S. Alamian

Department of Brucella, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

T. Zahraei Salehi

Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

K. Aghaiypour Kolyani

Department of Biotechnology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

M. Esmaelizad

Department of Biotechnology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

A. Etemadi

Department of Brucella, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

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