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مقالات فارسیISIکنفرانسهاژورنالها

Detection of Mycoplasma agalactiae in Small Ruminants of Southeast Iran

محل انتشار: مجله آرشیو رازی، دوره: 72، شماره: 4
سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 47

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https://civilica.com/doc/1868701

شناسه ملی سند علمی:

JR_ARCHRAZI-72-4_004

تاریخ نمایه سازی: 6 دی 1402

چکیده مقاله:

Agalactia is an infectious and contagious disease of small ruminants caused by Mycoplasma agalactiae (M. agalactiae). Although different microorganism strains contribute to this disease,  M. agalactiae is known as the most prominent causative agent. Therefore, this study aimed to investigate the rate of M. agalactiae involvement in contagious agalactia in the southeast region of Iran. Sampling was performed from milk, conjunctiva, ear lesions, and joints exudate of suspicious sheep and goat flocks according to the reports of Iran Veterinary Organization. The presence of Mycoplasma and its species, namely M. agalactiae, was evaluated through microbial culture and polymerase chain reaction (PCR) techniques. The detected microorganisms were confirmed to be Mycoplasma and M. agalactiae by the PCR amplification of ۱۶S rRNA and lipoprotein target genes. According to the findings of present study, ۱۴.۸% and ۳۶.۰% of the samples were diagnosed as positive for Mycoplasma by culture and PCR, respectively. Moreover, the incidence of M. agalactiae was determined as ۶.۱% using the specific PCR method. Therefore, it is recommended to identify the other species of Mycoplasma in small ruminant samples involved with contagious agalactiae disease.

کلیدواژه ها:

Agalactia ، Mycoplasma agalactiae ، PCR ، Southeast of Iran

نویسندگان

M. Shamsaddini Bafti

Department of Molecular Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Kerman, Iran

S.A. Pourbakhsh

Mycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran

M. Ezatkhah

Department of Molecular Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Kerman, Iran

A. Ashtari

Mycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran

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  • Hopert, A., Uphoff, C.C., Wirth, M., Hauser, H., Drexler, H.G., ...
  • Kheirabadi, K., Ebrahimi, A., ۲۰۰۷. Investigation of Mycoplasma agalactiae in ...
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  • Khezri, M., Pourbakhsh, S.A., Ashtari, A., Rokhzad, B., Khanbabaie, H., ...
  • Kumar, A., Rahal, A., Chakraborty, S., Verma, A.K., Dhama, K., ...
  • Mokhber Dezfouly, M., Sadeghian, S., Javanbakht, J., Lakzian, A., ۲۰۱۱. ...
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  • Quinn, P.J., Markey, B.K., Leonard, F.C., Hartigan, P., Fanning, S., ...
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