PCR-RELP for detecting of Theileria annulata infection in cattle and Hyalomma species in Kermanshah Province, Iran

Bovine theileriosis is important disease in tropical and subtropical areas with great economic losses in livestock husbandry in Iran. The aim of study was to assess the prevalence of Theileria annulata infection in cattle and Hyalomma species of Kermanshah Province, Iran. A number of ۱۳۸ blood samples were randomly taken from examined cattle. The genomic DNA was extracted and PCR was performed to specifically amplify a ۷۲۱-bp-long fragment of the ۳۰ Kilo Dalton major merozoite surface antigen (۳۰ KDa msa) of T. annulata. The amplified products were digested with TaqI, Rasl, and AluI restriction enzymes. Overall prevalence was ۹.۴۴% (۱۳/۱۳۸) with lymphadnopathy (۱.۱۷%) and pale mucosal membrane (۱.۹%) in Holstein cattle aged