An optimized affordable DNA-extraction method from Salmonella enterica Enteritidis for PCR experiments
محل انتشار: مجله آرشیو رازی، دوره: 68، شماره: 2
سال انتشار: 1392
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 44
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شناسه ملی سند علمی:
JR_ARCHRAZI-68-2_003
تاریخ نمایه سازی: 6 دی 1402
چکیده مقاله:
In diagnostic and research bacteriology settings with budget and staff restrictions, fast and cost-effective genome extraction methods are desirable. If not inactivated properly, cellular and/or environmental DNA nucleases will degrade genomic material during the extraction stage, and therefore might give rise to incorrect results in PCR experiments. When crude cell extracts, proteinase K–treated templates and purified DNAs prepared by phenol-chloroform-isoamylalcohol method as well as a commercial extraction kit were subjected to the Salmonella enterica Enteritidis specific STM۲ PCR, with exception of crude cell extract, PCR products from all other three methods saved their integrity for ۲۸ days post-generation. This work aimed to find out whether improvement to boiling method can guaranty stability of PCR products. As results showed, treatment of crude cell extracts from S. Enteritidis with proteinase K offers an inexpensive, fast and effective DNA extraction method suitable for high-throughput laboratories.
کلیدواژه ها:
نویسندگان
Rainak Ghaderi
Razi vaccine & Serum Research Institite AND Armenian National Agrarian University, Yerevan, Armenia
S. Moradi Bidhendi
Razi Vaccine & Serum Research Institute
P. Khaki
Razi Vaccine & Serum Research Institute
K. Tadayon
Razi Vaccine & Serum Research Institute
Nasim Karimnasab
Razi vaccine & Serum Research Institite AND Azad University-Karaj Branch
M. Sekhavati
Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran
F. Asadi
Razi Vaccine & Serum Research Institute