Comparative Study of Three Vaccinal Strains of Clostridium tetani Including Harvard ۵۲, G۵ and ۴۹۲۰۵ from Standpoint of Six Essential Factors to Evaluate Their Toxigenesis for Use in Tetanus Vaccine Production

Tetanus is an important disease which created by tetanospasmin toxin of Clostridium tetani. In this study we surveyed six important factors including LF, KF, MLD, pH, OD and total protein assay of Harvard ۵۲ (H۵۲), G۵ (HG۵) and ۴۹۲۰۵ (H۴۹۲۰۵) strains of the bacterium to determine which of them were more suitable for use in vaccine production. The mentioned strains were seperatedly reactivated in thioglycolate medium, ۲ml of this suspension was used to inoculate the Muller-Miller medium of each of sixteen ۵۰۰ ml Erlenmeyer flasks (۷,۶ and ۳ flasks for H۵۲, HG۵ and H۴۹۲۰۵ respectively), where the fermentation runs were performed. Over a period of seven days of experiment, several tests for evaluation of the six mentioned factors on samples of medium cultures were carried out. Results revealed that H۵۲ strain had significantly lower values in LF and OD compared to strains HG۵ & H۴۹۲۰۵ (P