Protective Effect of Curcumin on the Density of Hippocampal Dark Neurons in Mice Model of Aging Induced by D-galactose: A Histopathological Study
محل انتشار: مجله تحقیقات دارویی و بیومدیک، دوره: 5، شماره: 4
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 58
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شناسه ملی سند علمی:
JR_PBRE-5-4_007
تاریخ نمایه سازی: 10 دی 1402
چکیده مقاله:
Background: Curcumin is the most active ingredient in turmeric root of Curcuma longa of the Zingiberaceae family and has a potent antioxidant activity. This study aimed at investigating the effects of curcumin with various doses on the density of dark neurons in the hippocampus of induced D-galactose aging mice model. This experimental study was conducted on ۴۰ adult male BALB/c mice.
Materials and Methods: We randomly divided animals into ۵ groups: D-galactose, control, and curcumin ۱, curcumin ۲, and curcumin ۳ groups. D-galactose (۳۰۰ mg/kg) was intraperitoneally injected into the D-galactose group for ۶ weeks. D-galactose and doses of ۲۵, ۵۰, and ۱۰۰ mg of curcumin were administrated, respectively, to the curcumin groups ۱-۳ every day for ۱۴ days. After ۶ weeks, the mice’s brains were stained with toluidine blue after tissue passage. Then, the mean dark neuron volume density in each unit of the tissue was assessed using stereological formulas. The obtained data were analyzed by Aanlysis of variance (ANOVA) in SPSS.
Results: Compared with the control group, the average number of dark neurons in the hippocampus significantly increased following the administration of D-galactose (P=۰.۰۰۱). The average dark neurons frequency in the hippocampus significantly decreased in the ۵۰ and ۱۰۰ mg/kg curcumin-treated groups (P=۰.۰۰۱ for both) compared to that of the D-galactose treated animals.
Conclusion: The results of this study showed that treatment with ۱۰۰ mg/kg of curcumin reduced the number of dark neurons in the hippocampus of the aging mice. It seems that curcumin decreases dark neurons via the reduction of apoptosis. Also, curcumin is a powerful antioxidant and affects the level of antioxidant status in the brain.
کلیدواژه ها:
نویسندگان
Zahra Heidari
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Shabnam Mohammadi
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Mahdieh Yousefi Taba
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
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