Identification of drought stress-responsive long non-coding RNAs (lncRNAs) in root tip region of rice (Oryza sativa)
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 62
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شناسه ملی سند علمی:
JR_JPMB-10-2_003
تاریخ نمایه سازی: 20 دی 1402
چکیده مقاله:
Drought is a severe abiotic stress factor that significantly impacts rice production globally. The investigation of drought stress response components, particularly in plant roots, holds great importance. Recent evidence highlights the critical role played by long non-coding RNAs (lncRNAs) in the response to abiotic stress. In this study, we identified drought stress-induced lncRNAs in the root tip region of rice using transcriptome sequencing analysis performed on seedlings of a sensitive rice genotype (IR۶۴) under drought and control conditions. We identified a total of ۳۵۸ differentially expressed lncRNAs (DElncRNA), more than ۶۰% of which were in intergenic regions. Our results demonstrated that DElncRNAs can directly or indirectly regulate ۷۱۰ and ۷۵۳۵ mRNAs in cis and trans, respectively. Additionally, the target genes of DElncRNAs were involved in drought resistance genes, lateral root growth, and genes affecting auxin transport. We also identified ۲۴ conserved sequence motifs in the upstream regions of DElncRNAs and differentially expressed mRNAs (DEmRNAs) motifs through a search and functional analysis of these motifs indicated their involvement in regulation of transcription, translation, and the transmembrane receptor protein tyrosine kinase signaling pathway. Finally, we constructed a network of DElncRNAs and DEmRNAs. Our functional analysis of the top ۱۰ hub lncRNAs in the network demonstrated their involvement in growth processes, cellular responses to stimuli, and signaling pathways. These results offer a comprehensive perspective on potentially functional lncRNAs and provide insight into the molecular mechanisms underlying drought resistance in the root tip region of rice.
کلیدواژه ها:
نویسندگان
Sara Esmaeili Tazangi
Institute of Biotechnology, Shiraz University, Shiraz, Iran.
Ali Niazi
Institute of Biotechnology, Shiraz University, Shiraz, Iran.
Mohammad Reza Ghaffari
Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.
Abbas Alemzadeh
Department of Crop Production and Plant Breeding, School of Agriculture, Shiraz University, Shiraz
Ahmad Tahmasebi
Institute of Biotechnology, Shiraz University, Shiraz, Iran.
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