Evaluation of Iead Effects on Laccase Enzyme Activity in Bacillus Subtilis WPI

سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 53

فایل این مقاله در 6 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_REMJ-7-2_003

تاریخ نمایه سازی: 22 دی 1402

چکیده مقاله:

Background: : Lead (Pb) is an important environmental pollutants that play a significant role in increasing the stability of some other pollutants by changing the microbial profile of the soil.  Bacillus subtilis WPI is an abundant bacteria existing in wastewater. Because of laccase enzyme in this bacterium, the decomposition process of aromatic pollutants in wastewaters can be facilitated. We aimed to investigate the effect of different Pb concentrations on B. subtilis growth and biological activity of laccase enzyme in B. subtilis WPI. Material and Methods: B. subtilis WPI was isolated from the paper mill industrial wastewater of Hormozgan, Iran, from March to August ۲۰۱۷. After purification, the growth trend of B. subtilis WPI as well as the activity of laccase enzyme in different concentrations of Pb was investigated based on kinetic method. Results: Bacterial growth at Pb concentration of ۴۰۰ mg/L reduced in a dose-dependent manner, and this decrease was significant at concentrations of ۳۰۰ and ۴۰۰ mg/L (P < ۰.۰۰۱). The level of laccase enzyme activity in the lead concentration range of ۲۰-۱۶۰ mg/L also reduced in a dose-dependent manner, which implied that the highest decrease was observed at lead concentration of ۱۶۰ mg/L (P < ۰.۰۱). Our findings showed that there was no significant change in bacterial growth in lead concentration range of ۲۰-۲۰۰ mg/L, while a significant change was observed in the activity of laccase enzyme in the mentioned concentration range. Conclusion: Therefore, it seems that this reduction in enzyme activity can indirectly increase the stability of aromatic oil pollutants in the environment.

نویسندگان

Parisa Mehrabi Moghadam

Islamic Azad University, Hamadan Branch, Hamadan, Iran

Hassan Mahmoudi

Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • Buragohain M, Bhuyan B, Sarma HP. Seasonal variations of lead, ...
  • Wang C, Zhao M, Lu L, Wei X, Li T. ...
  • Oroojalian F, Kasra-Kermanshahi R, Azizi M, Bassami MR. Phytochemical composition ...
  • Ahalya N, Ramachandra T, Kanamadi R. Biosorption of heavy metals. ...
  • Ruijssenaars H, Hartmans S. A cloned Bacillus halodurans multicopper oxidase ...
  • Endo K, Hayashi Y, Hibi T, Hosono K, Beppu T, ...
  • Koschorreck K, Schmid RD, Urlacher VB. Improving the functional expression ...
  • Shraddha, Shekher R, Sehgal S, Kamthania M, Kumar A. Laccase: ...
  • Pazarlıoǧlu NK, Sariişik M, Telefoncu A. Laccase: production by Trametes ...
  • Xu F. Applications of oxidoreductases: recent progress. Industrial Biotechnology. ۲۰۰۵;۱(۱):۳۸-۵۰ ...
  • Sheikhi F, Ardakani MR, Enayatizamir N, Rodriguez-Couto S. The determination ...
  • Dalfard AB, Khajeh K, Soudi MR, Naderi-Manesh H, Ranjbar B, ...
  • Bains J, Capalash N, Sharma P. Laccase from a non-melanogenic, ...
  • Shirdam R, Khanafari A, Tabatabaee A. Cadmium, nickel and vanadium ...
  • Wang C-l, Zhao M, Li D-b, Cui D-z, Lu L, ...
  • Hullo M-F, Moszer I, Danchin A, Martin-Verstraete I. CotA of ...
  • Baldrian P, Gabriel J. Copper and cadmium increase laccase activity ...
  • Niku-Paavola ML, Karhunen E, Salola P, Raunio V. Ligninolytic enzymes ...
  • Cooper VS, Bennett AF, Lenski RE. Evolution of thermal dependence ...
  • Shukur YN. Determination of Optimal Conditions for the Production of ...
  • Wang J, Chen C. Biosorbents for heavy metals removal and ...
  • Ghezelbash FSMRANEG. Isolation and identification of two laccase producer fungi ...
  • Couto SR, Herrera L. Inhibitors of laccases: a review. Current ...
  • Lugauskas A, Levinskaitë L, Peèiulytë D, Repeèkienë J, Motuzas A, ...
  • Couto SR, Sanromán M, Gübitz G. Influence of redox mediators ...
  • Zhang H-B, Yang M-X, Shi W, Zheng Y, Sha T, ...
  • Mayer AM, Staples RC. Laccase: new functions for an old ...
  • نمایش کامل مراجع