Antibacterial Effects of Citrus aurantium on Bacteria Isolated from Urinary Tract Infection

Background :  Emerging antibacterial resistance rates and beta-lactamase producing bacteria recovered from UTI is an increasing problem in different regions, limiting therapeutic options. Therefore, this survey consider to use the extract and essence of the citrus aurantium (which have a so many rate of planting in Iran) and also survey on extract on bacteria whose cause urinary tract infections, and compare this with common antibiotics. Methods and Materials: This study was experimental design.We have been isolate the E.coli,Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae and Enterococcus faecalis from UTI and then determine of antibacterial effect of Citrus aurantium against this bacteria with subculture and put the exact diagnosis on them. Antibacterial effects of the herb extract by well diffusion assay and  nalidixic acid and Co-trimoxazol were evaluated by method of agar disc diffusion. Results:Enterococcus faecalis had ۱۰۰% sensitivity against of extract,essence and Co-trimoxazole , and ۸۰% against nalidixic acid . E.coli had ۱۰۰% sensitivity against Co-trimoxazol, nalidixic acid and it was totally resistance to extract and essence.Klebsiella Pneumonie had ۸۰% to Co-trimoxazol, ۷۵% to nalidixic acid and resistance against extract and essence.Streptococcus agalactiae was ۱۰۰% sensitivity to essence and Co-trimoxazol and ۹۰% against nalidixic acid and shown ۸۰% sensitivity against extract.Staphylococcus aureus MRSA shown ۱۰۰% sensitivity against Co-trimoxazol and ۷۰% sensitivity against essence, extract and nalidixic acid. Conclusion: Detection of antibiotic resistance among isolates is important in prevention and control of infections. In this study, it was shown that extracts of citrus aurantium have high antibacterial effects on gram positive bacteria compare to gram negative bacteria.