First Detection of ۱۶S rRNA Methylase and blaCTX-M-۱۵ Genes among Klebsiella pneumoniae Strains Isolated from Hospitalized Patients in Iran

Fatemeh Ashrafian; Fatemeh Fallah; Ali Hashemi; Soroor Erfanimanesh; Shokouh Amraei; Samira Tarashi

First Detection of ۱۶S rRNA Methylase and blaCTX-M-۱۵ Genes among Klebsiella pneumoniae Strains Isolated from Hospitalized Patients in Iran

محل انتشار: مجله تحقیق در پزشکی مولکولی، دوره: 3، شماره: 4

سال انتشار: 1394

نوع سند: مقاله ژورنالی

زبان: انگلیسی

مشاهده: 52

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https://civilica.com/doc/1882074

شناسه ملی سند علمی:

JR_REMJ-3-4_005

تاریخ نمایه سازی: 22 دی 1402

چکیده مقاله:

Background: The increasing pattern of Multi-Drug Resistant (MDR) bacteria has limited therapeutic options especially for nosocomial isolates of Klebsiella pneumoniae. Therefore, the aim of this study was the molecular detection of ۱۶S rRNA methylase and blaCTX-M-۱۵ among K. pneumoniae strains isolated from hospitalized patients in Mofid, Imam Hossein and Taleghani hospitals. Materials and Methods:This study was done with ۱۱۰ K.pneumoniae isolated of hospitals in Tehran, Iran. Antibiotic susceptibility tests were carried out by Kirby-Bauer disc diffusion and broth microdilution methods according to CLSI guidelines. ESBL, AmpC and KPC enzymes were detected by CDDT and MHT methods and the armA, rmtB, rmtC, rmtD and blaCTX-M-۱۵ genes were detected by PCR and sequencing techniques. Typing of antibiotic resistance isolates was carried out by PFGE technique. Results: In this study, Fosfomycin, colistin and tigecycline were more active than other antibiotics. Among the ۱۱۰ K. pneumoniae strains, ۶۰(۵۴.۵%), ۳۳(۳۰%) and ۵(۴.۵%) were ESBL, Amp-C and KPC positive, respectively. The existence of blaCTX-M-۱۵, armA and rmtC was detected in ۴۰(۳۶.۳%), ۱۵ (۱۳.۶%) and ۲ (۱.۸%) respectively. Of ۱۵ representative armA-producing K. pneumoniae isolates analyzed by PFGE, ۹ different pulsotypes (PF۱–۹) were identified with Dice coefficients of ;ge۹۰% similarity. Conclusions: High-level aminoglycoside resistance in human pathogens result of ۱۶S rRNA methylases is one of the serious concerns in Iran.

کلیدواژه ها:

Klebsiella pneumoniae ، ۱۶s rRNA methylase ، β-lactamases

نویسندگان

Fatemeh Ashrafian