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The potential of human-derived periodontal ligament stem cells to osteogenic differentiation: An In vitro investigation

محل انتشار: مجله تحقیق در پزشکی مولکولی، دوره: 2، شماره: 4
سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 42

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لینک ثابت به این مقاله:
https://civilica.com/doc/1882089

شناسه ملی سند علمی:

JR_REMJ-2-4_004

تاریخ نمایه سازی: 22 دی 1402

چکیده مقاله:

Background: Periodontal ligament stem cells (PDLSCs) are considered as a type of mesenchymal stem cell that is beneficial target for numerous clinical applications in periodontal tissue regeneration therapy. Materials and Methods: This study examined the effects of dexamethasone (Dex) on human PDLSCs in vitro. PDLSCs obtained from the roots of patient’s teeth were cultured with Dex (۰.۰۱ μM), and their proliferation was measured. The osteogenic differentiation was assessed by alkaline phosphatase (ALP) activity and Alizarin Red-S staining for calcium deposition. Results: After the administration of ۰.۰۱ μM Dex, the activity of ALP increased significantly. Furthermore, mineralized nodule formation showing the intracellular calcium deposition was significantly higher in the Dex-treated cells than that of the control cells. Conclusion: Collectively, Dex has positive effects on osteogenic differentiation of human PDLSCs in vitro. It is suggested that PDLSCs may serve as a potential material for periodontal tissue regeneration.

کلیدواژه ها:

Periodontal ligament stem cells ، Osteogenic differentiation ، Dexamethasone

نویسندگان

Behzad Houshmand

Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Omolbanin Amjadi

Molecular and Cell Biology Research Center, Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Mazandaran, Iran

Alireza Rafiei

Molecular and Cell Biology Research Center, Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Mazandaran, Iran

Mohammadali Rouzegar

Department of periodontology, School of Dentistery, Ilam University of Medical Sciences, Ilam, Iran

Mohammadreza Abrishami

Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mohammadreza Talebi Ardakani

Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

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