Effects of RF-EMF Exposure from GSM Mobile Phones on Proliferation Rate of Human Adipose-derived Stem Cells: An In-vitro Study

Background:As the use of mobile phones is increasing, public concern about the harmful effects of radiation emitted by these devices is also growing. In addition, protection questions and biological effects are among growing concerns which have remained largely unanswered. Stem cells are useful models to assess the effects of radiofrequency electromagnetic fields (RF-EMF) on other cell lines. Stem cells are undifferentiated biological cells that can differentiate into specialized cells. Adipose tissue represents an abundant and accessible source of adult stem cells. The aim of this study is to investigate the effects of GSM ۹۰۰ MHz on growth and proliferation of mesenchymal stem cells derived from adipose tissue within the specific distance and intensity.Materials and Methods:ADSCs were exposed to GSM mobile phones ۹۰۰ MHz with intensity of ۳۵۴.۶ µW/cm۲ square waves (۲۱۷ Hz pulse frequency, ۵۰% duty cycle), during different exposure times ranging from ۶ to ۲۱ min/day for ۵ days at ۲۰ cm distance from the antenna. MTT assay was used to determine the growth and metabolism of cells and trypan blue test was also done for cell viability. Statistical analyses were carried out using analysis of one way ANOVA. P