Combined Effect of Iodine Contrast Media, Cisplatin and External Beam Radiotherapy on Anaplastic Thyroid Cancer Cells

Introduction: The current study investigated the combination of high Z atoms (iodine-, platinium-based drugs) with using low energy irradiation (۱۲۰kvp) in Anaplastic Thyroid cancer cells.Material and Methods: For this purpose, eight groups were designed: control (CNT), different concentrations of Iodine contrast media (ICM), irradiation with various doses, Cis-platin (CDDP) with different concentrations, (ICM + CDDP), (ICM + RAD), (CDDP + RAD) and (ICM + CDDP + RAD). The viability was measured by MTT and Colony assay. In MTT assay, the viability of ۸۳۰۵c cells RAD (۲ Gy)+ICM (۱۰mg/mL) group was significantly lower than those treated with RAD or ICM alone. CDDP +ICM+RAD group significantly decreased the viability. In colony assay, cells in ICM + RAD (۲ Gy) group reduced the number of colonies more significant than RAD group. The difference of colony forming ability between CDDP and CDDP + RAD (۲ Gy) was significant. The difference of ICM + CDDP + RAD (۲ Gy) and CDDP +RAD (۲ Gy) group was significant. All data were statistically analysed using one-way analysis of variance (ANOVA) followed by Chafe’s multi-comparisons tests. All data were presented as mean ± standard deviation (SD) and analysed using statistical package for social sciences (SPSS ۱۶). Significance was considered to be p<۰.۰۵.Results: In MTT assay, the viability of ۸۳۰۵c cells RAD (۲ Gy) + ICM (۱۰mg/mL) group was significantly lower than those treated with RAD or ICM alone. CDDP + ICM + RAD group significantly decreased the viability. In colony assay, cells in ICM + RAD (۲ Gy) group reduced the number of colonies more significantly than RAD group. The difference of colony forming ability between CDDP and CDDP + RAD (۲ Gy) was significant. The difference of ICM + CDDP + RAD (۲ Gy) and CDDP + RAD (۲ Gy) group was significant.Conclusion: Exposure of ATC to ICM in the presence of CDDP increases tissue X-rays absorbance by Auger electrons and photo electrons leading to more fatal effects against the tumour.