Bystander Effect Induced in Breast Cancer (MCF-۷) and Human Osteoblast Cell Lines (hFOB ۱.۱۹) with HDR-Brachytherapy
محل انتشار: مجله فیزیک و مهندسی پزشکی، دوره: 10، شماره: 3
سال انتشار: 1399
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 45
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شناسه ملی سند علمی:
JR_JBPE-10-3_009
تاریخ نمایه سازی: 30 دی 1402
چکیده مقاله:
Background: Radiation induced bystander effects (RIBEs) occurs in unirradiated cells exhibiting indirect biological effect as a consequence of signals from other irradiated cells in the population. Objective: In this study, bystander effects in MCF-۷ breast cancer cells and hFOB ۱.۱۹ normal osteoblast cells irradiated with gamma emitting HDR Brachytherapy Ir-۱۹۲ source were investigated.Material and Methods: In this in-vitro study, bystander effect stimulation was conducted using medium transfer technique of irradiated cells to the non-irradiated bystander cells. Cell viability, reactive oxygen species (ROS) generation and colony forming assay was employed to evaluate the effect. Results: Results indicate that the exposure to the medium irradiated MCF-۷ induced significant bystander killing and decreased the survival fraction of bystander MCF-۷ and hFOB from ۱.۱۹ to ۸۱.۷۰ % and ۶۵.۴۴ %, respectively. A significant decrease in survival fraction was observed for hFOB ۱.۱۹ bystander cells (p < ۰.۰۵). We found that the rate of hFOB ۱.۱۹ cell growth significantly decreases to ۸۵.۵% when added with media from irradiated cells. The ROS levels of bystander cells for both cell lines were observed to have an increase even after ۴ h of treatment. Our results suggest the presence of bystander effects in unirradiated cells exposed to the irradiated medium. Conclusion: These data provide evidence that irradiated MCF-۷ breast cancer cells can induce bystander death in unirradiated MCF-۷ and hFOB ۱.۱۹ bystander cells. Increase in cell death could also be mediated by the ROS generation during the irradiation with HDR brachytherapy.
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نویسندگان
N H Mohd Zainudin
PhD student, Medical Radiation Programme, School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia
R Abdullah
PhD, Medical Radiation Programme, School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia
W N Rahman
PhD, Department of Nuclear Medicine, Radiotherapy and Oncology, Hospital Universiti Sains Malaysia, Kelantan, Malaysia
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