As a general defense system, the skin is the largest organ in the human body.The disruption of the integrity or malfunction of the skin tissue is generally called a wound. One of the wound dressings is biological wound dressings. Bioactive wound dressings include tissue engineering products from natural or synthetic sources. Nanotechnology-based platforms have gained a growing interest in wound healing. Metal nanoparticles, such as gold, exhibit attractive properties such as low toxicity in vivo and anti-microbial activity. Chemical methods typically leave some toxic reagents on the nanoparticles. For this reason, plants like Allium jesdianum as sustainable sources and used in the preparation of biocompatible
nanoparticles have attracted the attention of many researchers in recent years. Non-toxicity, biocompatibility, cheapness, and production of
nanoparticles with high purity can be mentioned from this method.Methods: To fabricate the nanofibers containing AuNPs, concentrations obtained from MIC Au
nanoparticles for each bacterium were added to the PAN solution. Thus, a scaffold containing ۰.۲% Au
nanoparticles was produced for S. aureus. A scaffold containing ۲۷% Au
nanoparticles was fabricated for E. coli. A scaffold containing ۸.۳% Au
nanoparticles was fabricated for P. aeruginosa. The morphology, size, and zeta potential of Au NPs were analyzed by TEM and DLS, respectively. FT-IR studied the surface functional groups of green synthesized nanoparticles. DPPH assays determined the antioxidant capacity of green synthesized nanoparticles.The migration capability was reflected by Scratch assay.Results: The nanocomposites exhibited a high antioxidant effect and antibacterial activity against S.aureus, E.coli, and P.aeruginosa. Besides, based on the results of the cell viability assays, the optimum concentration of AuNPs in the nanofibrous mats is ۰.۰۸% w/v and had no cytotoxicity on fibroblast cells. The scaffold also showed good blood compatibility according to the effects of coagulation time. As well as significant fibroblast migration and proliferation on the wound margin, according to wound-healing assay. The developed biocompatible, antioxidant, and antibacterial AuNPs incorporated in PAN nanofibrous mats showed their potential as an effective wound dressing. The results showed the prepared nanofibers adorned with Au
nanoparticles possess considerable antioxidant ability and synergic antimicrobial activity.