Evaluate the Effect of PI۳K Pathway Regulators with Melatonin on Arrested Human Embryo Development In vitro

Introduction: IVF is one of the methods used for assisted reproduction. According to studies, ۴۰ to ۵۰% of IVF Embryos cannot reach the blastocyst stage. Embryos stop on the third to fourth day after fertilization, and are divided into three groups: type ۱, which is ۲ to ۴ cells, type ۲, which is between ۴ and ۸ cells, And the third type, which is in the beginning. Morula stage, which is type ۱ in MZT and type II and III. They are disturbed at the level of glycolysis and phosphoryl oxidation. And they may be disturbed in the Signaling pathways. Signaling pathways can be regulated by antioxidants. In this study, it was investigated whether melatonin can regulate the pi۳k signaling pathway or not.Method: In this study, with the consent of the subjects, the collection of IR.ACECR.ROYAN.REC.۱۴۰۲.۰۰۸ samples from ۷۲-hour-old human embryos began after obtaining the ethical charter. This study was conducted in three main stages. The first step is melatonin dosing and choosing the best dose. The second stage is to culture the embryos of the control group and the treatment groups with the appropriate dose obtained. The third phase is blastocyst evaluation. Embryos including pluripotency and cell cycle genes were analyzed using the q-real-time PCR technique.Result: The analysis showed that the PI۳K pathway is one of the pathways involved in cell proliferation that can be regulated by melatonin. A melatonin antioxidant and supplement medium were used to prevent amage. melatonin with a concentration of ۰.۰۰۲ mmol causes a significant percentage of the aborted embryos to reach the blastocyst. After treatment, ۱۱/۳۵ embryos began to grow again. However, of the embryos that redeveloped (۹.۳۵, ۲۵%), only ۱ was compressed, and only (۶.۳۵, ۲۰%) reached the blastocyst stage. In the next step, we examined aging and cell cycle-related genes that increased the expression of pluripotency genes compared to the control groups. The expression of the cell cycle inhibitor CDKN۱A was also decreased. CDKNA۱, the cycle inhibitory gene, had a significant decrease in expression in the treatment groupConclusion: In fact, melatonin through the receptor Mt۱ activates the pi۳k signaling pathway and its downstream The Erk pathway is responsible for regulating six basic cellular processes: cell proliferation, cell division, and cell division. survival, cell growth, cell metabolism, cell migration, and cell differentiation. Melatonin can play a role in the develop.