Antibacterial Activity of Lucilia Sericata Extracts Against Wound Pathogens

Background: Wound infection is an ongoing problem and may result in prolonged hospital stay of patients and increased healthcare costs. Since Infected wounds with antibiotic resistant bacteria are responsible for significant human morbidity and mortality worldwide, it calls for introducing new alternative antimicrobial agents. The present study investigated antibacterial activity of different extracts of Lucilia sericata larvae against wound pathogens.Methods: Phosphate buffer saline (PBS) and different concentration of ethanol (۳۰%, ۵۰%, ۷۰%, and ۹۶%) were used for preparation of extracts from dried and frozen larvae of Lucilia sericata. The antibacterial activity of the extracts was assessed by disk diffusion and colony count techniques against Staphylococcus aureus isolates from wound samples. In all cases, solvents were individually tested as controls.Results: Only dried larval extract (with ۷۰% ethanol) showed significant antibacterial activities using disk diffusion method (the inhibition zone of ۱۷ mm against S. aureus isolate) and colony count techniques (۳ log reduction in the presence of the extract after ۲۴ hours).Conclusion: One of the prepared larval extracts showed significant antibacterial activity against wound pathogens and suggest new promise in treatment of wound infections. More investigations are needed for determination of MIC and MBC of this larval extract.