Assessment of the proliferative and angiogenic effects of the synthetic cannabinoid (R)-۵-fluoro ADB on human cerebral microvascular endothelial cells
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 27، شماره: 3
سال انتشار: 1403
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 50
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شناسه ملی سند علمی:
JR_IJBMS-27-3_006
تاریخ نمایه سازی: 8 بهمن 1402
چکیده مقاله:
Objective(s): The process of vascular formation, also known as angiogenesis, primarily relies on endothelial cell proliferation, migration, and invasion. In recent years, it has been discovered that synthetic cannabinoids (SCs) may potentially impact angiogenic processes within the body. We evaluated the impact of the synthetic cannabinoid (R)-۵-Fluoro-ADB on the proliferation rate and angiogenesis in Human Cerebral Microvascular Endothelial Cells (hBMECs). Materials and Methods: hBMECs were treated with (R)-۵-Fluoro-ADB and investigated for cell viability, migration rate, and tube-like structure formation. Furthermore, angiogenic-related proteins including Angopoitein-۱ and -۲, and Vascular Endothelial Growth Factors (VEGF) were examined on mRNA and protein levels.Results: The results showed a notable rise in the rate of proliferation (P-value<۰.۰۰۰۱) of HBMECs induced by (R)-۵-Fluoro-ADB. The angiogenic capacity of HBMECs was also enhanced between ۰.۰۰۱ μM to ۱ μM (R)-۵-Fluoro-ADB. Moreover, an increase in the levels of ANG-۱, ANG-۲, and VEGF mRNA and protein, as well as elevated phosphorylation rate of GSK-۳β, were observed across various concentrations of (R)-۵-Fluoro-ADB.Conclusion: Our results suggest an innovative approach in pharmacology for addressing a range of conditions linked to angiogenesis. This approach involves precise targeting of both cannabinoid receptors type-۱ and -۲. To achieve this, specific agonists or antagonists of these receptors could be employed based on the particular characteristics of the diseases in question.
کلیدواژه ها:
Brain ، Cannabinoids ، Endothelial cells ، Glycogen synthase kinase ۳ beta ، Vascular endothelial growth - factors
نویسندگان
Laith Al-Eitan
Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid ۲۲۱۱۰, Jordan
Saif Zuhair
Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid ۲۲۱۱۰, Jordan
Iliya Khair
Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid ۲۲۱۱۰, Jordan
Mansour Alghamdi
Department of Anatomy, College of Medicine, King Khalid University, Abha ۶۱۴۲۱, Saudi Arabia
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