Association of VEGF Gene rs۲۰۱۰۹۶۳ C/T Polymorphism with Idiopathic Asthenozoospermia in Iranian Azeri Males

سال انتشار: 1403
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 45

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شناسه ملی سند علمی:

JR_REMJ-10-4_004

تاریخ نمایه سازی: 14 بهمن 1402

چکیده مقاله:

Background: Infertility is one of the major issues in human reproduction, and male-related factors account for approximately half of all infertility cases. Genetic factors and single nucleotide polymorphisms (SNPs) are the most important factors contributing to a man's infertility. The aim of this study was to evaluate the relationship between vascular endothelial growth factor (VEGF) gene polymorphism (rs۲۰۱۰۹۶۳ C/T) and idiopathic infertility in Iranian Azeri men with asthenozoospermia (AZS). Materials and Methods: The study investigated ۴۰ men with idiopathic infertility (case group) and ۴۰ healthy men who had successfully conceived (control group) from the East Azerbaijan, Iran population. The genomic DNA was extracted and the genotype analysis was performed by tetra-primer amplification refractory mutation system-polymerase chain reaction (Tetra-ARMS PCR). To analyze the obtained data, statistical software was utilized. Results: Genotype frequency in infertile individuals was ۲۵% CC, ۵۶.۲۵ CT, and ۱۸.۷۵ TT, while ۳۰% CC, ۶۰% CT, and ۱۰% TT were in control subjects. In terms of genotype and phenotypic distribution, there was no statistically significant relationship between the case and control groups. Conclusion: Our research results indicated no significant relationship between the VEGF gene rs۲۰۱۰۹۶۳ C/T polymorphism and idiopathic AZS in Iranian Azeri males. However, greater sample counts and more precise research on various geographical locations, races, and ethnicities are crucial.

نویسندگان

Niloufar Bahadori

Department of Molecular Biology, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Masoud Maleki

Department of Molecular Biology, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Mahsan Azimidizaj

Department of Molecular Biology, Tabriz Branch, Islamic Azad University, Tabriz, Iran

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