the Genetic variation of ACTN۳ and MSTN genes in a cohort of endurance Arabian horses
محل انتشار: مجله جهانی تحقیقات علوم دامی، دوره: 7، شماره: 2
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 44
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شناسه ملی سند علمی:
JR_GJSAR-7-2_002
تاریخ نمایه سازی: 23 بهمن 1402
چکیده مقاله:
Arabian horses are readily distinguishable in form and features, and they are widely known for endurance capability. This study is the first examining of endurance-related genes in Arabian horses born and raised in Syria. The major objective was to identify genetic variation in candidate genes that could potentially affect endurance traits and to associate them with endurance phenotypes. The two genes Alpha-actinin skeletal muscle isoform ۳ (ACTN۳) and Myostatin (MSTN) were sequenced. Performance traits were available for ۴۲ recorded Arabian horses from Syria performed endurance racing over ۴۰, ۸۰, and ۱۲۰ km distances. Based on the recorded mean speeds, horses were grouped according to their performance index into low and high performers. The comparative sequencing revealed a total of ۱۳ variants in both studied genes, ۱۲ variants in ACTN۳ and one variant in MSTN. General linear model analyses showed that none of the analyzed variants has significant effect on any of the studied traits. However, for ACTN۳, we found a ۵’ UTR variant (۱۲:۲۶۵۱۱۷۰۴G>A) that predicted to cause a gain of an E۴BP۴ transcription factor binding site, and a variant in the ۳´ UTR ۱۲:۲۶۵۲۴۹۳۰T>C that predicted to cause the abrogation of two predicted miRNA target sites (eca-miR-۱۲۹۶ and eca-miR-۳۲۶) and thereby affect gene expression. For MSTN, a ۵’ UTR variant ۱۸:۶۶۴۹۵۶۹۶A>G is predicted to cause the substitution of the transcription factor binding sites for HFH-۱ and Sox-۵ by binding sites for HFH-۳ and E۴BP۴.Arabian horses are readily distinguishable in form and features, and they are widely known for endurance capability. This study is the first examining of endurance-related genes in Arabian horses born and raised in Syria. The major objective was to identify genetic variation in candidate genes that could potentially affect endurance traits and to associate them with endurance phenotypes. The two genes Alpha-actinin skeletal muscle isoform ۳ (ACTN۳) and Myostatin (MSTN) were sequenced. Performance traits were available for ۴۲ recorded Arabian horses from Syria performed endurance racing over ۴۰, ۸۰, and ۱۲۰ km distances. Based on the recorded mean speeds, horses were grouped according to their performance index into low and high performers. The comparative sequencing revealed a total of ۱۳ variants in both studied genes, ۱۲ variants in ACTN۳ and one variant in MSTN. General linear model analyses showed that none of the analyzed variants has significant effect on any of the studied traits. However, for ACTN۳, we found a ۵’ UTR variant (۱۲:۲۶۵۱۱۷۰۴G>A) that predicted to cause a gain of an E۴BP۴ transcription factor binding site, and a variant in the ۳´ UTR ۱۲:۲۶۵۲۴۹۳۰T>C that predicted to cause the abrogation of two predicted miRNA target sites (eca-miR-۱۲۹۶ and eca-miR-۳۲۶) and thereby affect gene expression. For MSTN, a ۵’ UTR variant ۱۸:۶۶۴۹۵۶۹۶A>G is predicted to cause the substitution of the transcription factor binding sites for HFH-۱ and Sox-۵ by binding sites for HFH-۳ and E۴BP۴.
کلیدواژه ها:
نویسندگان
Saria Almarzook
Humboldt University of Berlin , Germany
ammar Said Ahmed
Humboldt University of Berlin , Germany
Monika Reissmann
Humboldt University of Berlin , Germany
Gudrun Brockmann
Humboldt University of Berlin , Germany
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