Correlation between staining intensity of prostatic adenocarcinoma with P۵۳ and Gleason's grading

Introduction: Prostatic adenocarcinoma is the most common malignancy of internal system and after lung cancer is the second cause of death in male. Genetic base of many neoplasms is related to suppressor tumor gene such as P۵۳. Therefore, we aimed in this study, to assess the intensity of prostatic adenocarcinoma tissue staining with P۵۳ staining method and its relationship with Gleason grade. Methods: In this cross-sectional study, during ۲۰۰۶ to ۲۰۰۸, all patients attending pathology ward of Shahid Mohammadi hospital with diagnosed prostatic adenocarcinoma enrolled for the study. Samples were stained with H & E staining and if adenocarcinoma was confirmed they stained with P۵۳ stain using immunohistichmistry (IHC) method and severity of staining was recorded. We used colon cancer tissue for positive control and non tumoral prostate tissue for negative control. Data were analyzed using descriptive method and Chi-square test by means of SPSS. Results: ۳۰ patients aged between ۶۰ and ۹۱ with mean age of ۷۷.۱۰±۸.۵ enrolled in the study. Grade of tumors vary from ۲ to ۱۰ and the most frequent grade was ۷. Results show that with increase in the Gleason grade, intensity of P۵۳ staining also increases. (P<۰.۰۰۱) Conclusion: Results demonstrated that there is a direct relationship between Gleason grade and intensity of P۵۳ staining.