Exploring the Correlation between Expression of EMT Transcription Factors and CXCR۴ Expression

Background: Metastasis is one the most leading cause of death from cancer. The chemokine receptor of CXCR۴ has an important role in cell migration and cancer metastasis. Additionally, metastasis is always associated with the process of epithelial-mesenchymal transition (EMT). In this study the correlation between expression of CXCR۴ and EMT-TFs has been examined.Methods: The expression of CXCR۴ in knocked out SUM۱۵۹ cell line for EMT-TFs of slug, snail, twist and ZEB۱ were examined.Results: The results revealed that the expression of CXCR۴ decreased significantly in twist and ZEB۱ knocked out cells, however in other groups no change was observed. Decreased expression of CXCR۴ indicated that ZEB۱ and twist may be one of regulators of CXCR۴ expression.Conclusions: ChIP assay should be performed in future experiments to see the definite role of ZEB۱ and twist as transcription factors for CXCR۴.Background: Metastasis is one the most leading cause of death from cancer. The chemokine receptor of CXCR۴ has an important role in cell migration and cancer metastasis. Additionally, metastasis is always associated with the process of epithelial-mesenchymal transition (EMT). In this study the correlation between expression of CXCR۴ and EMT-TFs has been examined. Methods: The expression of CXCR۴ in knocked out SUM۱۵۹ cell line for EMT-TFs of slug, snail, twist and ZEB۱ were examined. Results: The results revealed that the expression of CXCR۴ decreased significantly in twist and ZEB۱ knocked out cells, however in other groups no change was observed. Decreased expression of CXCR۴ indicated that ZEB۱ and twist may be one of regulators of CXCR۴ expression. Conclusions: ChIP assay should be performed in future experiments to see the definite role of ZEB۱ and twist as transcription factors for CXCR۴.