Phenotypic Detection of Extended-Spectrum β-lactamases (ESBLs) and Aminopenicillin Cephalosporinase (AmpC)-Producing Bacterial Isolates from Surfaces of Hospital Fomites and Hands of Healthcare Workers
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 41
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شناسه ملی سند علمی:
JR_JMMI-11-3_004
تاریخ نمایه سازی: 29 بهمن 1402
چکیده مقاله:
Introduction: The hospital environment can significantly contribute to the spreading of bacterial isolates that pose a risk to public health. In this study, we analyzed bacteria found on hospital fomites and the hands of healthcare workers to determine the presence of resistant enzymes such as ESBLs and AmpC. Methods: We studied ۱۰۰ samples collected from hospital fomites - including the hands of healthcare workers - for bacterial growth, which were subsequently identified using standard procedures. Standard disk methods were used to screen Gram-negative bacteria (GNB) for ESBL and AmpC production, including presumptive and confirmatory testing. Results: ۴۶ (۴۶.۰%) Gram-negative bacteria were isolated from all sampling sites, including a preponderance of Pseudomonas aeruginosa and Escherichia coli. Of the ۴۶ GNBs, ۳۱ (۶۷.۴%) and ۲۷ (۵۸.۷%) were resistant to ceftazidime and ceftriaxone, respectively. The double disk synergy test (DDST) showed ESBL in ۳۴ (۷۳.۱%) of the isolates, with the highest prevalence in E. coli (۳۲.۳%) and P. aeruginosa (۲۶.۵%). These isolates were primarily associated with patients’ bedding (۳۲.۴%), tablets (۲۶.۵%), and sinks (۲۰.۶%), although there was no statistical difference (P=۰.۹۹۸). Presumptive AmpC production was ۱۰۰% in isolates of K. pneumoniae, C. diversus, Shigella spp., and S. marcescens but variable in other isolates. The combined disk test (CDT) showed that ۲۹ (۶۳.۰%) isolates were AmpC-producing GNB, with the highest prevalence in E. coli (۳۴.۵%). Conclusion: The isolation of bacteria with these types of resistance from the surfaces of hospital fomites may negatively impact the quality of healthcare delivery.
کلیدواژه ها:
نویسندگان
Musa Y. Tula
Department of Biological Science Technology, Federal Polytechnic Mubi, Adamawa State, Nigeria
Osaretin Iyoha
Department of Medical Microbiology, School of Medicine, College of Medical Sciences, University of Benin, P.M.B. ۱۱۵۲, Benin City, Nigeria
Richard Elisha
Department of Pharmaceutical and Biomedical Technology, Federal Polytechnic Mubi, Adamawa State, Nigeria
Joel Filgona
Department of Microbiology, Adamawa State University Mubi, Nigeria
Abumhere S. Aziegbemhin
Department of Microbiology, Faculty of Life Sciences, University of Benin, PMB ۱۱۵۴ Ugbowo, Benin City, Edo State Nigeria
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