Prevalence of Extended Spectrum β-Lactamases-Producing Escherichia coli Isolated from Clinical Samples and Their Antibiotic Resistance Pattern
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 35
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شناسه ملی سند علمی:
JR_JMMI-11-1_004
تاریخ نمایه سازی: 29 بهمن 1402
چکیده مقاله:
Introduction: Infections caused by ESBL-producing bacteria are a growing concern worldwide, especially in developing countries like Nigeria. Hence, this study aimed to isolate, screen, and identify ESBL-producing Escherichia coli from clinical samples and analyze their antibiotic resistance patterns. Methods: ۲۰۰ clinical samples were collected, consisting of ۶۰ stool, ۸۸ urine, and ۵۲ wound pussamples. We used the pour-plate method on MaCconkey agar (MAC) for isolation. After suspected E. coli was isolated, we sub-cultured it on eosin methylene blue (EMB) agar. To confirm E. coli identification, we used cultural and biochemical assays. Disc and double disc diffusion methods were employed to screen and confirm ESBL-producing E. coli. Antimicrobial susceptibility testing was conducted by disc diffusion technique, and the results were interpreted using CLSI standards. Results: A total of ۴۷ E. coli isolates were obtained, with ۴۸.۵% of the isolates originating from urine samples. These isolates were among six different genera of bacteria. Among the E. coli isolates, ۱۶ were confirmed to be ESBL producers. The ESBL-producing E. coli demonstrated high resistance to amoxicillin-clavulanic acid (۸۷.۵%), ampicillin (۷۵.۰%), and cefotaxime (۵۰.۰%). Before plasmid curing, the bacteria demonstrated a high susceptibility to chloramphenicol (۸۱.۳%) and amikacin (۵۶.۳%). However, varying antibiotic resistance and susceptibility degrees were observed after plasmid curing. Conclusion: ESBL-producing E. coli showed a high resistance level (۳۴.۰%) against most discs used. However, chloramphenicol and amikacin showed promise as potential treatments for ESBL-producing E. coli infections. In addition, it is recommended that clinical laboratories should include routine ESBL detection methods for ongoing surveillance of antibiotic-resistant isolates. This will serve as a helpful guide for empirically treating bacterial infections.
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نویسندگان
Mohammed Ja'afaru
Department of Microbiology, Modibbo Adama University, Yola, PMB ۲۰۷۶, Adamawa State, Nigeria
Abubakar Gaure
Department of Microbiology, Modibbo Adama University, Yola, PMB ۲۰۷۶, Adamawa State, Nigeria
Joel Ewansiha
Department of Microbiology, Faculty of Life Sciences, Modibbo Adama University, Yola
Olumuyiwa Adeyemo
Department of Biotechnology, Modibbo Adama University, Yola, PMB ۲۰۷۶, Adamawa State, Nigeria
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