Burkholderia cepacia Complex, an Emerging Nosocomial Pathogen at Health Care Facilities in Sebha, Libya
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 43
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شناسه ملی سند علمی:
JR_JMMI-9-4_001
تاریخ نمایه سازی: 29 بهمن 1402
چکیده مقاله:
Introduction: Burkholderia cepacie complex (Bcc) is an emerging multidrug-resistant gram-negative bacteria frequently isolated from health care facilities worldwide. The present study investigated the prevalence of Bcc in health care settings in Sebha, Libya. Methods: Two hundred swabs were initially collected. Forty-seven nosocomial Bcc isolates were identified from three medical care facilities, i.e., ۴۰ (۲۰%) from Sebha Medical Center, five from the Sebha Infertility Treatment Center, and two from Althanweya Clinic. The isolates were identified using a combination of biochemical tests and USP chapter <۶۰> Microbiological Examination of Non-Sterile Products Tests for Burkholderia Cepacia Complex guidelines. A UPGMA dendrogram was used to examine the biochemical relationship of isolates. Some of the putative virulence factors contributing to the pathogenicity of the isolates were also explored. Results: Of the ۴۷ isolates, ۲۹.۷۹% were B. cepcia, ۲۳.۴۰% B. cenocepcia, ۱۲.۷۷% B. thailandensis, ۸.۵۱% B. vietnamiensis, ۶.۳۸% B. ambifaria, B. pyrrocinia, and B. stabilis each, ۴.۲۶% B. anthina, and ۲.۱۳% B. arboris. A variation in virulence factors was observed among isolates; all (۱۰۰%) isolates produced siderophore, ۹۱% had capsules, ۹۱% produced lipase, ۸۹% formed a biofilm, and ۴۹% produced alkaline protease. The UPGMA dendrogram revealed that Bcc species shared substantial phenotypical identity among themselves. Conclusion: In developing countries with limited resources, diagnostic challenges in identifying Bcc species can be resolved using selective media and USP chapter <۶۰> guidelines.
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نویسندگان
Shamsi Saad Shamsi
Department of Plant Science, Microbiology Program, Research laboratory, Faculty of Science, Sebha University
Abdelkader A. Elzen
Department of Microbiology, Faculty of Science, Sebha University, Sebha, Libya
Khadija M. Ahmad
Department of Microbiology, Faculty of Medicine, Sebha University, Sebha, Libya; Department of Microbiology, Sebha medical center, Sebha
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