Antibacterial activity of porous alumina nanoparticles against ۱۰ clinical isolates of Staphylococcus aureus

BACKGROUND AND OBJECTIVESStaphylococcus aureus is one of the most important bacteria causing infections in humans. The prevalence of these infections, along with the increase in antibiotic-resistant strains, has become a concerning problem. The use of nanoparticles to combat bacterial infections can serve as an alternative to antibiotics. The aim of this study was to investigate the therapeutic effect of alumina nanoparticles on Staphylococcus aureus isolated from clinical samples, considering the growth of antibiotic resistance and exploring new approaches in nanobiotechnology.MATERIALS AND METHODSIn this study, ۱۰ strains of Staphylococcus aureus isolated from clinical samples were examined based on catalase, coagulase, and mannitol fermentation tests for identification and diagnostic purposes. The antibacterial activity was evaluated using the well diffusion method on Mueller-Hinton agar culture medium. Different concentrations of nanoparticles were prepared in equal volumes of dimethyl sulfoxide and methanol solvent. After incubation at ۳۷ ℃for ۲۴ hours, the sensitivity of bacteria was determined by measuring the diameter of the growth inhibition zone, and the minimum inhibitory concentration was determined.RESULTS AND DISCUSSIONAll isolates of Staphylococcus aureus showed sensitivity to alumina nanoparticles, and the average minimum inhibitory concentration was found to be ۲۰ mg/ml.CONCLUSIONThe effectiveness of alumina nanoparticles against clinical strains of Staphylococcus aureus indicates that this substance can be considered as a suitable antimicrobial agent.