Isolation and detection of Brucella species from patients suspected to brucellosis in North Khorasan, Iran

BACKGROUND AND OBJECTIVESBrucellosis, caused by various species of the genus Brucella, remains a significant public health concern globally. Timely and accurate diagnosis of this infectious disease is crucial for effective management and control. In this study, titled "Isolation and detection of Brucella species from patients suspected to brucellosis," the researchers aimed to isolate and identify Brucella species among patients presenting with suspected brucellosis.MATERIALS AND METHODSBlood samples were obtained from patients suspected to Brucellosis. Serum was separated and used for ۲me and wright test. Also, blood samples were cultured on brucella agar and incubated in ۷۲°C for ۱ week. Bio typing and phage typing tests using were performed for positive samples.RESULTS AND DISCUSSIONAmong ۲۰۰ suspected patients, ۵۰ were serologically positive. Also, blood culture of ۵ patients tested positive. Bio typing revealed that all positive samples were infected with Brucella melitensis biovar ۱.CONCLUSIONThe study successfully isolated and identified Brucella species among patients suspected to have brucellosis. The combination of serological testing, bacterial culture, and molecular methods allowed for accurate detection and characterization of the pathogen. The predominance of Brucella melitensis biovar ۱ suggests a specific strain responsible for human brucellosis in the North Khorasan population. These findings contribute to our understanding of the epidemiology and help guide appropriate treatment and prevention strategies for brucellosis.