Biofilm formation and cell adhesion of Acinetobacter baumannii ATCC ۱۹۶۰۶ and its clinical isolation on HeLa cervical epithelial cells in the presence and absence of anti-Oma۸۷ antibody and acute cytotoxicity of antigen

BACKGROUND AND OBJECTIVESBacterial resistance, especially resistance to Acinetobacter baumannii, has become one of the main problems of the medical community in the world today. Bacteria of the genus Acinetobacter are gram negative, completely aerobic, catalase positive and oxidase negative and due to continuous mutations and many resistances, they are considered one of the most important causes of human infections, especially in hospital environments.MATERIALS AND METHODSAfter expressing and purifying the outer membrane protein of Acinetobacter baumannii called oma ۸۷ and injecting it into mice at different time intervals, blood was drawn from the mice in order to prepare anti-oma۸۷ antibody and with the resulting serum, which contains a significant amount of anti-antibody oma۸۷, biofilm, toxicity, attachment and internalization tests were performed. For example, the biofilm test was conducted in such a way that the amount of biofilm formation by two strains of Acinetobacter baumannii, one of which was a standard strain and the other a clinical strain, was checked in the presence and absence of mouse serum containing anti-oma۸۷ antibody. Therefore, in the same way, the effect of serum containing antibody on the toxicity of Acinetobacter baumannii cells, the degree of adhesion of bacteria to the cell, and the entry of bacteria into the host cell were also performed on the mentioned strains, just like the biofilm test.RESULTS AND DISCUSSIONAfter conducting the tests, it was found that the anti-Oma۸۷ antibody is able to prevent the pathogenesis of A. baumannii bacteria.